| 含巯基氨基酸促血管内皮细胞氧化的对照研究 |
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| 引用本文： | 苏娟,秦燕,钮荣祥,吴建新.含巯基氨基酸促血管内皮细胞氧化的对照研究[J].医学理论与实践,2012,25(6):623-624. |
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| 作者姓名： | 苏娟 秦燕 钮荣祥 吴建新 |
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| 作者单位： | 云南大理学院基础医学院生理学与病理生理学教研室,云南省大理,671000 |
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| 基金项目： | 云南省教育厅科学研究基金项目 |
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| 摘 要： | 目的:探讨不同含巯基氨基酸对人血管内皮细胞的氧化作用。方法:用含有1 000μmol/L的同型半胱氨酸(Hcy)、半胱氨酸(Cys)、还原型谷胱甘肽(GSH)培养液培养细胞24h后,以分光光度比色法分别测定细胞内丙二醛(MDA)含量和超氧化物酶(SOD)活力的改变。结果:Hcy可促使细胞内MDA含量的增加和SOD活力的下降。Cys和GSH对细胞内MDA的含量无影响。Cys可促进细胞内SOD活力的升高。结论:Hcy通过氧化应激促进血管内皮细胞的损伤。
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| 关 键 词： | 同型半胱氨酸 半胱氨酸 谷胱甘肽 氧化应激 |
A Comparative Study of the Oxidative Injury of Endothelial Cells by Thiol Amino Acids |
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| Institution: | SU Juan,QIN Yan,NIU Rongxiang,et al.Department of Physiology and Pathophysiology,School of Basic Medicine,Dali University,Dali,Yunnan Province 671000 |
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| Abstract: | Objective:This study was intended to detail the effects of three thiol amino acids(Hcy,Cys and GSH) on the oxidation of endothelial cells.Methods:Endothelial cells were incubated with Hcy,Cys and GSH(1 000μmol/L)for 24 hours.The contents of MDA and the activities of SOD were measured by chromatometry.Results:Hcy significantly increased the level of MDA and decreased the activities of SOD.Cys increased the activities of SOD.Conclusion:Hcy injuried the endothelial cells through oxidative stress. |
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| Keywords: | Homocysteine Cysteine Glutathione Oxidative stress |
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