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对氧磷酶长循环脂质体的制备及评价
引用本文:韩振坤,孙建波,刘丹,胡海洋,陈大为,顾鹏毅,赵敏.对氧磷酶长循环脂质体的制备及评价[J].中国医科大学学报,2010,39(2):87-91.
作者姓名:韩振坤  孙建波  刘丹  胡海洋  陈大为  顾鹏毅  赵敏
作者单位:1. 中国医科大学,附属盛京医院急诊科,沈阳,110004
2. 沈阳药科大学,药学院,沈阳,110016
3. 中国医科大学,实验技术中心,沈阳,110001
基金项目:国家自然科学基金资助项目(30671778); 辽宁省教育厅高校科研基金资助项目(05L480)
摘    要: 目的研制对氧磷酶长循环脂质体。方法采用薄膜分散法制备对氧磷酶长循环脂质体,采用凝胶柱法测定包封率,以包封率为指标,分别考察药脂比、胆固醇用量、聚乙二醇-胆固醇用量、离子强度等对脂质体的影响,在此基础上用正交设计对处方进行优化。结果经薄膜分散法制得的脂质体包封率为(87.66±3.46)%,平均粒径为126nm左右,粒度分布均匀,呈单峰分布,电镜结果显示外形圆整,分散性较好。4℃放置15d包封率无明显变化,酶活性基本稳定。结论聚乙二醇修饰的对氧磷酶长循环脂质体制备工艺简单可行,对氧磷酶长循环脂质体制剂学、酶学性质稳定。

关 键 词:对氧磷酶  长循环脂质体  薄膜分散法  蛋白  粒度分布

Preparation and Evaluation of Long-circulating Liposomes of Paraoxonase
HAN Zhen-kun , SUN Jian-bo , LIU Dan , HU Hai-yang , CHEN Da-wei , GU Peng-yi , ZHAO Min.Preparation and Evaluation of Long-circulating Liposomes of Paraoxonase[J].Journal of China Medical University,2010,39(2):87-91.
Authors:HAN Zhen-kun  SUN Jian-bo  LIU Dan  HU Hai-yang  CHEN Da-wei  GU Peng-yi  ZHAO Min
Institution:HAN Zhen-kun1,SUN Jian-bo1,LIU Dan2,HU Hai-yang2,CHEN Da-wei2,GU Peng-yi3,ZHAO Min1(1.Department of Emergency,Shengjing Hospital,China Medical University,Shenyang 110004,China,2.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,3.Technology Central Laboratory,Shenyang 110001,China)
Abstract:Objective To prepare the long-circulating liposomes of paraoxonase(PON).Methods The long-circulating liposomes of paraoxonase were prepared by film dispersion method.The encapsulation efficiency was determined by gel column.The effects of the factors on the encapsulation efficiency,such as the weight ratio of paraoxonase to phospholipid,cholesterol(Chol)to phospholipid,PEG-cholesterol(PEG-Chol)and the iron strength of water phase,were investigated respectively.Then the formulation was optimized by orthogona...
Keywords:paraoxonase  long-circulating liposome  film dispersion method  protein  partical size distribution  
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