HER-2 siRNA对卵巢癌细胞顺铂耐药性的影响

目的:观察表皮生长因子受体2(HER-2)siRNA转染SKOV-3细胞抑制HER-2表达后对卵巢癌细胞耐药性的影响.方法:体外转录合成HER-2序列特异性双链RNA(dsRNA),转染SKOV-3细胞株中,RT-PCR方法检测转染前后HER-2基因的mRNA的表达,MTT法检测顺铂对转染前后卵巢癌细胞的生长抑制率.结果:HER-2siRNA转染72 h后,对SKOV-3细胞HER-2 mRNA表达明显抑制.在不同浓度顺铂(0.05～50μg/ml)的作用下,转染HER-2 siRNA与转染非特异性siRNA、空白对照组相比,差异有十分显著性意义(P＜0.01).细胞对顺铂的敏感性约提高10倍.结论:体外转录合成的siRNA能有效抑制SKOV-3细胞中HER-2的表达,提高细胞对顺铂的敏感性,RNA干扰技术为卵巢癌的治疗提供了一种新策略.

Effect of HER-2 siRNA on the drug resistance of ovarian carcinoma SKOV-3 cells to cisplatin

Objective: To study the effect of HER-2 siRNA on the drug resistance of ovarian carcinoma SKOV-3 cells to cisplatin.Methods: HER-2 siRNA was synthesized in vitro and then transfected into SKOV-3 cells.The expression of HER-2 mRNA both before and after transfection was detected by RT-PCR.The chemosensitivity of transfected and untransfected cells to cisplatin was detected by MTT method.Results: Sequence specific siRNA targeting HER-2 down-regulated the expression of HER-2 mRNA markedly 72 hours after the transfection.In the presence of different concentrations of cisplatin,there were significant differences both between HER-2 siRNA transfected group and non-specific siRNA transfected group and between HER-2 siRNA transfected group and control group(P<0.01).The chemosensitivity of HER-2 siRNA transfected cells to cisplatin increased by 10 times.Conclusion: Sequence specific siRNA targeting HER-2 could inhibit the expression of HER-2 in SKOV-3 cells and thus increase the sensitivity of the cells to cisplatin.RNA interference provides a new strategy to the treatment of ovarian carcinoma.