氟西汀和噻萘普汀对慢性应激抑郁模型大鼠海马Bcl-2表达的影响

 目的 研究抗抑郁药物氟西汀与噻萘普汀对慢性应激抑郁模型大鼠海马神经元Bcl-2蛋白表达的影响。方法 将大鼠随机分为抑郁模型组、氟西汀组、噻萘普汀组和对照组。模型组、氟西汀组和噻萘普汀组给予21 d的应激刺激，此期间对照组正常饲养，刺激期间氟西汀组每天灌胃氟西汀（10 mg/kg），噻萘普汀组每天灌胃噻萘普汀（50 mg/kg），模型组和对照组每天灌胃等体积的生理盐水。行为学检测应用开场法和液体消耗实验。采用Western-blot法检测各组大鼠海马神经元Bcl-2蛋白的表达情况。结果 应激后模型组水平穿越格数、竖立次数、修饰次数、糖水消耗百分比均显著低于对照组（P0.05）。在Western-blot检测中，慢性应激后模型组大鼠海马Bcl-2的表达水平显著低于对照组（P0.05）；噻萘普汀组大鼠海马Bcl-2的表达水平高于模型组（P0.05）。结论 慢性应激可能导致大鼠海马Bcl-2表达降低，氟西汀和噻奈普汀可能逆转慢性应激抑郁模型大鼠海马中Bcl-2表达的降低。

ffects of Fluoxetine and Tianeptine on Expression of Bcl-2 in Hippocampus of Chronic Stress Depression Rats

Objective To research the effects of fluoxetine and tianeptine on expression of Bcl-2 in hippocampus of chronic stress depression rats.Methods All the experimental rats were divided by random into 4 groups:Group of control,Group of depression,Group of fluoxetine and Group of tianeptine.The rats of Group of depression,Group of fluoxetine and Group of tianeptine were applied stress for 21 days,and meanwhile Group of control no stress.The rats of Group of fluoxetine were fed with fluoxetine,Group of tianeptine were fed with tianeptine,while another groups were fed with normal sodium.The expression of Bcl-2 was detected by Western-blotting method.Results In Western-blotting method,the level of Bcl-2 in the hippocampus of Group of depression is less than that of Group of control(P < 0.01);that of Group of fluoxetine is more than that of Group of depression(P < 0.01) and no obvious difference with Group of control(P > 0.05);that of Group of tianeptine is more than that of Group of depression(P < 0.01) and less than Group of control but no obvious difference(P > 0.05).Conclusion The expression of Bcl-2 in hippocampus of chronic stress depression rats decreased and could be reversed by fluoxetine and tianeptine.