| 颌下腺导管细胞体外分离纯化的实验研究 |
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| 引用本文： | 黄绍辉,谭学新,周青,邓春富,王玉新.颌下腺导管细胞体外分离纯化的实验研究[J].中国医科大学学报,2004,33(4):335-336. |
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| 作者姓名： | 黄绍辉 谭学新 周青 邓春富 王玉新 |
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| 作者单位： | 中国医科大学附属第一医院口腔颌面外科,辽宁,沈阳,110001 |
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| 摘 要： | 目的:体外分离纯化颌下腺(SMG)导管细胞.方法:选用8 d龄SD大鼠15只,无菌条件下取出颌下腺,胰蛋白酶消化,等密度梯度离心分离纯化导管细胞,免疫组织化学染色进行细胞鉴定,分光光度计检测所获得的各层细胞带上清液中淀粉酶的活性.结果:所获得的上层区带细胞免疫组织化学染色Anti-Pan cytokeratin、Anti-EMA呈阳性表达.同时淀粉酶活性检测上、下层区带分别为6%和72%.结论:应用等密度梯度离心的方法可获得纯化程度较高的SMG导管细胞.
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| 关 键 词： | 颌下腺导管细胞 等密度梯度离心 分离纯化 |
| 文章编号： | 0258-4646(2004)04-0335-02 |
| 修稿时间： | 2003年10月31 |
Isolation and purification of ductal cells of submandibular glands in vitro |
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| HUANG Shao-hui,TAN Xue-xin,ZHOU Qing,DENG Chun-fu,WANG Yu-xin.Isolation and purification of ductal cells of submandibular glands in vitro[J].Journal of China Medical University,2004,33(4):335-336. |
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| Authors: | HUANG Shao-hui TAN Xue-xin ZHOU Qing DENG Chun-fu WANG Yu-xin |
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| Abstract: | Objective: To isolate and purify ductal cells of submandibular glands (SMG) in vitro. Methods: The ductal cells of SD rats were isolated with density-gradient centrifugation under aseptic condition. Immunohistochemistry staining was used to determine the cells. The level of isolation and purification was evaluated by assay of amylase activity. Results: The cells in upper zone were positively stained with Anti-Pan cytokeratin and Anti-EMA,which were ductal specific antibodies. The activity of amylase of cells in upper and lower zones were 6% and 72% respectively. Conclusion: Pure ductal cells could be obtained with density-gradient centrifugation. |
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| Keywords: | ductal cell of submandibular gland density-gradient centrifugation isolation and purification |
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