| 一步离子交换层析法提纯牛视网膜可溶性抗原 |
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| 引用本文： | 柳力敏,陈蕾.一步离子交换层析法提纯牛视网膜可溶性抗原[J].中国医科大学学报,2002,31(6):413-415. |
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| 作者姓名： | 柳力敏 陈蕾 |
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| 作者单位： | 中国医科大学第一临床学院眼科,辽宁,沈阳,110001 |
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| 基金项目： | 辽宁省科学技术计划资助项目 (9680 2 0 0 5 ) |
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| 摘 要： | 目的：探讨一步离子交换层析法提纯牛视网膜可溶性抗原（S-Ag)。方法：利用DEAESepharoseFF凝胶及自动层析系统一步层析分离提纯牛视网膜S-Ag，聚丙烯酰胺凝胶电泳确定分子量，等电聚焦电泳确定等电点。结果：分析证实，S-Ag分子量52000，等电点6.20，利用提纯S-Ag成功地诱发出实验性自身免疫性葡萄膜视网膜类（EAU）动物模型。结论：经一步层析法提纯的S-Ag，可用于EAU动物模型的建立。
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| 关 键 词： | 可溶性抗原 自身免疫病 自身免疫性葡萄膜视网膜炎 一步离子交换层析法 提纯 牛视网膜可溶性抗原 |
| 文章编号： | 0258-4646(2002)06-0413-03 |
| 修稿时间： | 2001年7月19日 |
Purification of Soluble-Antigen From Bovine Retina by One-Step Ion Exchange Chromatography |
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| LIU Li min,CHEN Lei.Purification of Soluble-Antigen From Bovine Retina by One-Step Ion Exchange Chromatography[J].Journal of China Medical University,2002,31(6):413-415. |
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| Authors: | LIU Li min CHEN Lei |
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| Abstract: | Objective: Our aim was to isolate and purify soluble antigen (S Ag) from bovine retina by one step ion exchange chromatography. Methods: S Ag was isolated from bovine retina by ion exchange chromatography with DEAE sepharose fast flow and automatic gradient mixer system. The isolated S Ag was analyzed with sodium dodecyl sulfate polyacrylamide gel electrophoresis and isoelectric focusing. Results: The molecular weight of the purified S Ag is 52 000, and its isoelectric point is 6.20. The purified S Ag successfully induced the model of experimental autoimmune uveoretinitis (EAU). Conclusion: S Ag purified by one step ion exchange chromatography can be used for induction of EAU model. |
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| Keywords: | soluble antigen autoimmune disease experimental autoimmune uveoretinitis |
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