视网膜下液促使视网膜色素上皮细胞膜上蛋白激酶C变化的机制分析

目的：研究视网膜下液（SRF）能否引起体外培养的视网膜色素上皮（RPE）细胞浆内蛋白激酶C（PKC）的激活和转位，探讨SRF与RPE细胞中PKC信号系统变化的关系。方法：实验对象为体外培养的RPE细胞；不同的刺激因素分别在不同的时间刺激RPE细胞，通过细胞裂解和离心获取细胞浆和细胞膜蛋白粗提液，用同位素^32P标记和液体闪烁计数法检测细胞浆和细胞膜PKC活性水平。结果：SRF和佛波酯（PMA）都可以激活RPE细胞浆中的PKC（c-PKC），并使其由胞浆向胞膜转位，但胞膜上PKC（m-PKC）活性峰值水平和出现的时间不同。结论：SRF可引起RPE细胞膜上PKC的活性发生变化，变化的幅度与增殖性玻璃体视网膜病变（PVR）的分级呈正相关。

Change of protein kinase C activity induced by subretinal fluid in the cell membrane of retinal pigment epithelial cells

Objective: To investigate whether subretinal fluid(SRF) could induce the activation and translocation of protein kinase C(PKC) in the cytoplasm of retinal pigment epithelial(RPE) cells.Methods: RPE cells cultured in vitro were stimulated with different agents at different times.The PKC activity in cytoplasm and cell membrane was measured with radioactive isotope 32P label method.Results: Both SRF and phorbol 12-myristate 13-acetate(PMA) activated PKC in the cytoplasm,and PKC in the cytoplasm was translocated into the cell membrane.The occurrence time and level of the maximal activity of PKC in the cytoplasm differed.Conclusion: SRF can induce the activation and translocation of PKC in RPE cells,and the activity of PKC positively correlates with the degree of proliferative vitreoretinopathy.