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| 斑蝥素通过MAPK途径对肺癌A549细胞周期阻滞及其分子机制的研究 | |
| 引用本文： | 张卫东,赵惠儒,于秉治,王晓华,宗志红,刘莹.斑蝥素通过MAPK途径对肺癌A549细胞周期阻滞及其分子机制的研究[J].中国医科大学学报,2006,35(4):382-384. |
| 作者姓名： | 张卫东 赵惠儒 于秉治 王晓华 宗志红 刘莹 |
| 作者单位： | 1. 中国医科大学附属第一医院胸外科,辽宁,沈阳,110001 2. 基础医学院生物化学与分子生物学教研室 3. 肿瘤研究所肺癌研究室 |
| 摘 要： | 目的：探讨斑蝥素对人肺癌A549细胞的周期阻滞作用及其分子机制。方法：采用MTT法检测斑蝥素对A549细胞的增殖抑制作用；流式细胞仪检测细胞周期；以蛋白印迹法分析斑蝥素作用后细胞周期蛋白B1（cyelinB1）、p34^cdc2、phos-p34^cdc2、p21、survivin、ERK1／ERK2、phos-ERK1／phos-ERK2等蛋白表达或活性的改变。结果：斑蝥素能抑制A549细胞的增殖；斑蝥素作用于A549细胞后引起G2／M期阻滞；斑螯素处理后，p34^cdc2、phos-p34^cdc2。表达量没有改变，cyclinB1、survivin蛋白表达量减少，p21蛋白表达量增加。ERK1／ERK2、phos-ERK1／phos-ERK2表达量降低。结论：斑蝥素通过调节cyclinB1、p21、survivin、ERK1／ERK2、phos-ERK1／phos-ERK2等蛋白表达或活性的改变引起G2／M期阻滞。 |
| 关 键 词： | 斑蝥素 MAPK途径 细胞周期 肺癌A549细胞 |
| 文章编号： | 0258-4646(2006)04-0382-03 |
| 收稿时间： | 2005-04-21 |
| 修稿时间： | 2005年4月21日 |
Role and molecular mechanism of cantharidin in cell cycle arrest of A549 human lung carcinoma cells through mitogen-activated protein kinase pathway |
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| ZHANG Wei-dong,ZHAO Hui-ru,YU Bing-zhi,WANG Xiao-hua,ZONG Zhi-hong,LIU Ying.Role and molecular mechanism of cantharidin in cell cycle arrest of A549 human lung carcinoma cells through mitogen-activated protein kinase pathway[J].Journal of China Medical University,2006,35(4):382-384. | |
| Authors: | ZHANG Wei-dong ZHAO Hui-ru YU Bing-zhi WANG Xiao-hua ZONG Zhi-hong LIU Ying |
| Institution: | 1. Department of Thoracic Surgery, The First Affiliated Hospital, China Medical University, Shenyang 110001, China; 2. Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences; 3. Laborary of Lung Cancer, Cancer Research Institute |
| Abstract: | Objective: To investigate the role and the molecular mechanism of cantharidin in inducing cell cycle arrest of A549 human lung carcinoma cells through mitogen-activated protein kinase(MAPK) pathway.Methods: MTT assay was performed to determine the inhibitory effect of cantharidin on the proliferation of A549 cells.Fluorescence activated cell sorter (FACS) was used to detect the cell cycle.The changes in the expressions of cyclinB1,p34~(cdc2),phos-p34~(cdc2),p21,survivin,ERK1/ERK2,and phos-ERK1/phos-ERK2 were determined by Western blotting.Results: Cantharidin inhibited the proliferation of A549 cells and arrested the cell cycle at G2/M phase.Cantharidin increased the expression of p21 and reduced the expressions of cyclinB1,survivin,ERK1/ERK2,and phos-ERK1/phos-ERK2,but no changes in the expressions of p34~(cdc2) and phos-p34~(cdc2) were found.Conclusion: Cantharidin may arrest the cell cycle of A549 cells mainly by regulating the expressions of cyclinB1,p21,survivin,ERK1/ERK2,and phos-ERK1/phos-ERK2. |
| Keywords: | catharidin mitogen-activated protein kinase pathway cell cycle |
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