口腔舌鳞癌p53基因突变及其表达的相关性研究

目的：探讨口腔舌鳞癌中p53基因突变及其蛋白的表达两者相关性。方法：应用Western blot和聚合酶链反应-单链构象多态性方法检测38例口腔舌鳞癌、20例口腔癌前病变及10例正常口腔黏膜组织中p53蛋白表达及p53基因突变情况。结果：p53蛋白在口腔舌鳞癌、癌前病变和正常口腔黏膜组织的阳性表达率分别为42．1％（16／38）、10．0％（2／20）、0％（0／10）。39．5％（15／38）的口腔舌鳞癌存在p53基因第5、8外显子突变。在p53基因突变的病例中，p53蛋白表达与其基因突变密切相关。结论：p53基因突变及其蛋白过量表达与口腔舌鳞癌的发生、发展密切相关。

Clinical significance of p53 mutation and p53 protein expression in oral tongue squamous cell carcinoma

Objective: To investigate the relationship of p53 mutation and p53 protein expression in oral tongue squamous cell carcinoma.Methods: Western blot and polymerase chain reaction-single strand conformation polymorphism were performed to determine p53 mutation and p53 protein expression in 38 cases of oral tongue squamous cell carcinoma,20 cases of precancerous lesion,and 10 normal oral mucosal tissues.Results: The positive expression rates of p53 protein were 42.1%(16/38),10%(2/20),and 0%(0/10) in oral tongue squamous cell carcinoma,precancerous lesion,and normal oral mucosal tissues,respectively(P < 0.05).Mutation in exons 5 and 8 of the p53 gene was found in 39.5%(15/38) of oral tongue squamous cell carcinom.p53 mutation was closely related to p53 protein overexpression(P < 0.05).Conclusion: p53 mutation and p53 protein overexpression play important roles in the occurrence and development of oral tongue squamous cell carcinoma.