自发性2型糖尿病动物模型KKAy小鼠肾脏损害的特征与演变

 摘要: 目的 观察自发性2型糖尿病动物模型KKAy小鼠糖尿病早期肾损害的特征和演变，旨在探讨其在糖尿病肾病研究中的应用价值。方法 选择8周龄雄性KKAy小鼠和C57BL/6小鼠各20只，分别于8周龄、20周龄测定血糖、尿微量白蛋白、尿肌酐，并留取肾脏标本，于光镜及电镜下观察其肾脏病理特点。结果 ①不同周龄KKAy小鼠体重、随机血糖均高于对照组C57BL/6小鼠(P<0.05)。② 20周龄KKAy小鼠尿白蛋白排泄率显著高于8周龄KKAy小鼠和对照组C57BL/6小鼠(P<0.05)。③20周龄KKAy小鼠，光镜下肾小球面积增大, 肾小球毛细血管基底膜（GBM）增厚，系膜基质增生，可见硬化结节；电镜下肾小球基底膜增厚，足突部分扁平、融合。结论 20周龄KKAy小鼠出现肥胖、高血糖、尿白蛋白排泄率增加及典型的糖尿病肾病病理改变，是研究2型糖尿病早期肾损害的理想动物模型。

Renal Impairment in KKAy Mice with Spontaneous Type 2 Diabetes

Objective To investigate the early renal impairment in KKAy mice with spontaneous type 2 diabetes.Methods Blood glucose,urinary microalbumin and urinary creatinine of twenty male KKAy mice and twenty male C57BL/6 mice were measured at the age of 8,20 weeks respectively.The renal pathological changes were observed under light microscope and electron microscope.Results KKAy mice developed higher body weight and blood glucose than C57BL/6 mice at all the different ages(P < 0.05).Urinary microalbumin/creatinine...