缺血预适应对无复流现象的保护作用及机理的实验研究

目的：探讨缺血预适应（ＩＰＣ）对缺血再灌注（Ｉ／Ｒ）损伤无复流现象的保护作用及可能机理。方法：应用离体大鼠心脏全心Ｉ／Ｒ损伤模型，２０只Ｗｉｓｔａｒ大鼠被随机分为Ｉ／Ｒ对照组和ＩＰＣ组，观察冠脉流出液中ＭＤＡ及ＮＯ含量、无复流区域所占面积百分比、心脏微循环等指标的变化。结果：ＩＰＣ组较Ｉ／Ｒ对照组冠脉流出液中ＮＯ含量显著增高（Ｐ＜０．０１），ＭＤＡ含量明显降低（Ｐ＜０．００５），无复流区域所占面积百分比明显减少（Ｐ＜０．０００５），心脏微循环的损伤也较轻。ＮＯ含量和ＭＤＡ含量有明显负相关关系（ｒ＝－０．９０９，Ｐ＜０．０１）。结论：ＩＰＣ对无复流现象有保护作用，可能与其抗氧化、保护内源性ＮＯ释放有关。

The Protective Effect and Mechanism of Ischemic Preconditioning on No Reflow Phenomenon in Isolated Rat Hearts

Obijective: This paper was to study the protective effects and possible mechanism of ischemic preconditioning (IPC) on no reflow phenomenon. Methods: We established isolated rat heart model with ischemia and refusion (I/R) injury. Twenty wistar rats were randomly assigned to I/R control group and IPC group. Methylenedioxyamphetamine (MDA) and nitric oxide (NO) content in coronary effluent fluid, area of no reflow (%) and heart microcirculation were detected or observed during the course of I/R in control and ischemia preconditioned isolated rat hearts. Results: NO content in coronary effluent fluid was significantly higher in IPC group than that in I/R control group ( P <0 01), MDA content was significantly lower in the former than in the latter ( P< 0.0005). A negative correlation existed between MDA and NO content ( r=-0 909, P< 0 01). Area of no reflow (%) significantly decreased ( P< 0.0005). Heart microcirculation injury ameliorated. Conclusion: IPC may protect no reflow phenomenon by antioxidation and restraining the endogenous NO release.