表皮生长因子受体及Ki67在大肠肿组织中的表达及意义

目的 评价表皮生长因子受体（EGFR）及Ki67在大肠良、恶性肿瘤不周组织类型中的表达情况。方法 采用免疫组织化学SP法检测内镜及病理确诊的大肠息肉、大肠腺瘤和大肠腺癌患者共115例，分别对所选标本进行EGFR、Ki67检测，对其阳性表达率进行统计学分析。结果 EGFR、Ki67阳性反应分别主要定位干细胞膜、细胞核。息肉组EGFR、Ki67的阳性表达率均较低，分别为40．2％、12．3％。EGFR、Ki67在管状腺瘤及绒毛状腺瘤中阳性表达率均较高，管状腺瘤、绒毛状腺瘤中EGFR表达率分别为98．3％、70．4％，而Ki67在两者中表达率分别为78．6％、70．4％。EGFR及Ki67在管状腺癌、乳头状腺癌和粘液腺癌中的阳性表达率均较高（〉60％）。EGFR阳性表达在腺瘤与腺癌组织中无显著性差异（P〉O．05），而Ki67阳性表达在两者间有显著性差异（P〈0．001）。结论 EGFR、Ki67表达均与息肉无关。Ki67阳性表达比EGFR对大肠恶性肿瘤的诊断意义更大。

Expression of epidermal growth factor receptor and Ki67 in the colorectal tumors

Objective: To value the relevance of expressions of epidermal growth factor receptor(EGFR), Ki67 in different histological types of colorectal tumors. Methods: EGFR,Ki67 were detected by immunohistochemistrical SP method in 115 cases of colorectal polyps, adenoma and adenocarcinoma confirmed by endoscopy and pathology. The results were analyzed statistically. Results:EGFR and Ki67 were expressed in cell membrane and nucleus respectively. The expressions of EGFR, Ki67 in colorectal adenoma and adenocarcinoma were higher than those in the group of polyps. The expression of EGFR was higher in both tubular adenoma and tubulovillous adenoma, but even higher in the former.The expressions of EGFR and Ki67 were high (>60% ) in all types of adenocarcinoma.The expressive intensity has no significant difference between adenoma and adenocarcinoma(P>0.05),whereas that has significant difference between adenoma and adenocarcinoma(P<0.001). Conclusion:The expressions of EGFR, Ki67 have no significant relevance with polyps. The expression of Ki67 was more useful for early diagnosis in colorectal malignant tumors than the pression of EGFR.