| 荧光活性染料DiO在观察成骨诱导脂肪干细胞在脱钙骨材料上的生长与分布中的应用 |
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| 引用本文： | 万光勇,张明宾.荧光活性染料DiO在观察成骨诱导脂肪干细胞在脱钙骨材料上的生长与分布中的应用[J].泰山医学院学报,2011,32(9):649-652. |
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| 作者姓名： | 万光勇 张明宾 |
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| 作者单位： | 泰山医学院附属泰山医院口腔科,山东 泰安,271000 |
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| 摘 要： | 目的探讨荧光活性染料DiO对人脂肪干细胞（hADSCs）染色标记的效果及标记后的hADSCs在部分脱钙骨（PDBM）上的生长粘附情况。方法利用扫描电镜观察了细胞材料复合后细胞的生长情况；检测了DiO标记的hADSCs和DiO未标记的hADSCs在PDBM上粘附率，采用激光共聚焦显微镜观察观察了DiO标记的hADSCs在平面培养及在PDBM上的生长、粘附情况。结果DiO标记的hADSCs平面培养时可以良好地生长，DiO标记的hADSCs粘附率为（94．5±2．2）％，未标记的hADSCs粘附率为（95．1±1．3）％，统计学分析两者无显著差异（P〉0．05）。DiO标记的hADSCs在PDBM支架材料上可以良好的粘附生长。结论DiO对被标记细胞的存活、生长无影响、不影响细胞粘附，能反映细胞在支架材料上的正常生长状况。DiO标记是一种简洁、准确的观察hADSCs细胞在支架材料上粘附及生长状况的方法。
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| 关 键 词： | DiO 人脂肪干细胞（hADSCs） 部分脱钙骨（PDBM） 粘附率 激光共聚焦 |
Application of Dio to observe the osteogentic differentiated human adipose-derived stem cells on partially demineralized hone matrix |
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| WAN Guang-yong,ZHANG Ming-bin.Application of Dio to observe the osteogentic differentiated human adipose-derived stem cells on partially demineralized hone matrix[J].Journal of Taishan Medical College,2011,32(9):649-652. |
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| Authors: | WAN Guang-yong ZHANG Ming-bin |
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| Institution: | WAN Guang-yong ZHANG Ming-bin (Dept.of Oral and Dentofacial Surgery,Taian Central Hospital,Shangdong Province 271000,China) |
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| Abstract: | Objective: To investigate the effect of Dio on labeled hADSCs and its growth on partially demineralized bone matrix(pDBM).Methods: The growth of Dio-labeled hADSCs on pDBM was observed with Scanning Electron Microscope(SEM) and Laser Scanning Confocal Microscope(LSCM) respectively,and then the adhesion rate of Dio-labeled hADSCs and unlabeled ones were tested.Results: Dio-labeled cells grew fine in vitro,and there was no significant difference between the Dio-labeled cells and unlabeled ones in adhesion rat... |
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| Keywords: | Dio human adipose derived stem cells(hADSCs) partially demineralized bone matrix(pDBM) adhesion rate laser scanning confocal microscope(LSCM) |
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