| 胶原酶灌注法制备小鼠肝细胞 |
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| 引用本文： | 赵粤萍,周云,张军,刘广超,马远方.胶原酶灌注法制备小鼠肝细胞[J].河南大学学报(医学版),2008,27(3). |
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| 作者姓名： | 赵粤萍 周云 张军 刘广超 马远方 |
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| 作者单位： | 1. 河南大学,天然药物与免疫工程省级重点实验室,河南,开封,475003
2. 河南大学,免疫学研究所,河南,开封,475003 |
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| 基金项目： | 河南省高校杰出科研创新人才工程项目，河南省医学重大科技攻关计划项目 |
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| 摘 要： | 目的:建立体外培养小鼠肝细胞的实验体系。方法:采用两步原位胶原酶灌注法分离小鼠肝细胞,在RPM I1640培养液中培养。结果:用胶原酶灌注法分离的小鼠肝细胞活率可达90%以上,产量平均为2.8×107,完全满足实验要求。肝细胞在RPM I1640培养液中2 h即可贴壁,48 h内细胞贴壁充分,伸展良好,1周后死细胞增多。结论:胶原酶灌注法成功制备出小鼠肝细胞。
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| 关 键 词： | 胶原酶 肝细胞 分离 |
On the production of mouse hepatocytes by perfusion with collagenase |
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| ZHAO Yue-ping,ZHOU Yun,ZHANG Jun,LIU Guang-chao,MA Yuan-fang.On the production of mouse hepatocytes by perfusion with collagenase[J].Journal of Henan University,2008,27(3). |
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| Authors: | ZHAO Yue-ping ZHOU Yun ZHANG Jun LIU Guang-chao MA Yuan-fang |
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| Institution: | ZHAO Yue-ping,ZHOU Yun,ZHANG Jun,LIU Guang-chao,MA Yuan-fang*(1.Naturally Occurring Drugs , Immuno-engineering Key Laboratory of Henan University,Kaifeng 475003,China,2.Institute of Immunology,Henan University,China) |
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| Abstract: | Objective: To establish experimental system of mouse hepatocytes culture in vitro.Methods: Mouse hepatocytes were isolated by a two-step perfusion with collagenase in situ,and cultured in RPMI1640.Results: The cells viability(90%) and yield(×107) could meet the requirement of experiment.The cells attachment was noted after 2 h incubation in RPMI1640.During the following 48h,the cells attached completely,and extended well,but the death cells increased one week later.Conclusion: Mouse hepatocytes can be prepared by perfusion with collagenase successfully. |
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| Keywords: | Collagenase Hepatocyte Isolation |
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