| 蓖麻种子毒蛋白抗癌活性及热稳定性的探讨 |
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| 引用本文： | 张洪,敬永升,陈元,王聪,刘纯纯,郭书杰,李申,王富茹,王洪涛.蓖麻种子毒蛋白抗癌活性及热稳定性的探讨[J].河南大学学报(医学版),2011,30(1):47-50. |
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| 作者姓名： | 张洪 敬永升 陈元 王聪 刘纯纯 郭书杰 李申 王富茹 王洪涛 |
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| 作者单位： | 1. 河南大学生命科学学院生物工程研究所,河南省重点学科动物转基因与细胞工程开放实验室,河南,开封,475004
2. 河南大学药学院分析化学实验室,河南,开封,475004 |
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| 基金项目： | 国家自然科学基金，河南省高校创新人才资助计划 |
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| 摘 要： | 目的:了解蓖麻毒蛋白(Ricin)及从大戟科植物蓖麻种子中提取出来的一种核糖体失活蛋白的热稳定性和抗肿瘤活性.方珐:利用亲和层析的方法纯化蓖麻种子毒蛋白,并通过SDS-PAGE电泳和MTT染色法等方法检测其热稳定性、抗肿瘤性.结果:Ricin在37℃的缓冲液中保温60 h,没有明显的降解;能有效地杀死食管癌细胞EC10...
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| 关 键 词： | 核糖体失活蛋白 抗癌活性 稳定性 |
Study on the antitumor activity and the heat stability of Ricin |
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| ZHANG Hong,JING Yong-sheng,CHEN Yuan,WANG Cong,LIU Chun-chun,GUO Shu-jie,LI Shen,WANG Fu-ru,WANG Hong-tao.Study on the antitumor activity and the heat stability of Ricin[J].Journal of Henan University,2011,30(1):47-50. |
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| Authors: | ZHANG Hong JING Yong-sheng CHEN Yuan WANG Cong LIU Chun-chun GUO Shu-jie LI Shen WANG Fu-ru WANG Hong-tao |
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| Institution: | ZHANG Hong1,JING Yong-sheng2,CHEN Yuan1,WANG Cong2,LIU Chun-chun1,GUO Shu-jie1,LI Shen1,WANG Fu-ru1,WANG Hong-tao1(1.Institute of Bioengineering,College of Life Science,Henan University,Kaifeng,Henan 475004,China,Open Lab of Key Animal Transgenics and Cell Engineering Subjects of Higher Education of Henan Province Kaifeng,2.Analytical chemistry departmant,Pharmaceutical College of Henan University,China) |
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| Abstract: | Objective:To study the antitumor activity and the heat stability of Ricin,a kind of the ribosome-inactivating proteins(RIP) isolated from castor bean,euphorbia.Methods:Ricin was purified by affinity chromatography on Sepharose 4B,its heat stability and antitumor activity were respectively detected by SDS-PAGE and MTT method.Results: Ricin can exist in buffer solution for 60 hours without degradation at 37 ℃ and can kill esophageal cancer cells EC109.Conclusion: Ricin is a kind of protein which has a broad prospect of antitumor. |
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| Keywords: | RIP Antitumor activity Stability |
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