新安固本培元方对脾虚型溃疡性结肠炎大鼠细胞因子的影响

目的:观察固本培元方对脾虚型溃疡性结肠炎大鼠模型细胞因子的影响。方法:将60只大鼠随机分为空白组、模型组、SASP组(柳氮磺吡啶组)、固本培元方高、中、低剂量组6组,每组10只。除空白组外其余大鼠用番泻叶与冰乙酸造模,SASP组予以柳氮磺吡啶灌胃,高、中、低剂量组给予新安固本培元方煎剂灌胃,空白组及模型组灌服等量生理盐水。观察各组大鼠症状、体征,计算结肠黏膜形态损伤评分,检测血清IL-6、IL-8、IL-10及TNF-α的浓度。结果:模型组大鼠血清IL-6、IL-8及TNF-α的浓度及结肠黏膜形态损伤评分较空白组明显升高,IL-10含量显著降低(P0.01);与模型组比较,SASP组、高、中、低剂量组血清IL-6、IL-8及TNF-α的浓度及结肠黏膜形态损伤评分均明显下降(P0.01),且高剂量组降低TNF-α、减轻结肠黏膜损伤的效果优于中、低剂量组(P0.05)。结论:新安固本培元方对脾虚型大鼠溃疡性结肠炎有较好的治疗作用,适合在临床上应用和推广。

Effect of(Xin'an)Guben Peiyuan recipe on cytokines of rats with ulcerative colitis

Objective：To observe the effect of （Xin＇an） Guben Peiyuan recipe on cytokines of rats with ulcerative colitis （spleen deficient）. Methods ：60 rats were randomly divided into blank group,model group,SASP group（sulfasalazine group）, Guhen Peiyuan recipe high dose group.middle dose group, low dose group. Ulcerative colitis （spleen deficient syndrome） rat models were induced with folium sennae and aceticacid. The physical signs,symptoms of rats were observed and score of morphological damage of mucous membrane was calculated. The contents of IL-6, IL-8, IL-10 and TNF-α in serum were detected. Results：Compared with blank group,contents of IL-6,IL-8,TNF-α in serum and score of morphological damage of mucous membrane were increased,content of IL-10 was decreased in model group （P〈O.01）. Compared with model group,contents of IL-6,IL-8,TNF-α in serum and score of morphological damage of mucous membrane were increased in SASP group,high dose group,middle dose group and low dose group （P〈0.01）. The effect of high dose on regulating TNF-α,repairing mucous membrane was better than that of middle dose and low dose （P〈0.05）. Conclusion： Guhen Peiyuan recipe has curative effect on ulcerative colitis rats （spleen deficient）. It is suitable for clinical application.