基于代谢组学分析研究支气管哮喘的维医“同病异证”性

目的：揭示支气管哮喘患者异常黑胆质型与非异常黑胆质型的血浆代谢组变化情况及发生机制。方法对61例异常黑胆质型支气管哮喘患者和55名非异常黑胆质型支气管哮喘患者的血浆进行核磁共振氢谱检测，通过谱图分段积分后运用正交偏最小二乘判别分析法（OPLS-DA）分析所采集的图谱。结果异常黑胆质型哮喘患者与健康对照组相比，血浆中异亮氨酸、亮氨酸、丙氨酸、缬氨酸、酪氨酸、甘氨酸等氨基酸以及谷氨酰胺、α-葡萄糖、β-葡萄糖、糖蛋白、肌酸、乳酸、肉碱、丙酮、丙酮酸、脂类包含低密度脂蛋白（LDL）和极低密度脂蛋白（VLDL）]、不饱和脂类的含量均有不同程度的降低（P ＜0．05）。与非异常黑胆质型哮喘患者相比，异常黑胆质型哮喘患者血浆中α-葡萄糖、β-葡萄糖、糖蛋白明显升高（P ＜0．05），丙氨酸、谷氨酸、缬氨酸、亮氨酸、异亮氨酸、甘氨酸、酪氨酸、苯丙氨酸、1-甲基组氨酸等多种氨基酸含量升高，乙酰乙酸、丙酮，丙酮酸、乳酸、肌酸、肌酸酐、肉碱等代谢物含量升高，LDL 和 VLDL、不饱和脂类、乙酸、乙酰半胱氨酸、谷氨酰胺、鲨肌醇等化合物含量升高（P ＜0．05）。结论异常黑胆质型哮喘患者体内能量代谢和蛋白质代谢发生改变，体内糖异生和免疫功能的紊乱程度较严重，这可能是异常黑胆质型哮喘形成的生物学基础之一，也从一个侧面证实维医“同病异证”的科学性。

Different syndromes in the same disease of bronchial asthma of Uygur Medicine Abnormal Savda Syndrome Based on Metabonomics

Objective To explore the metabolic variation and pathogenesis of bronchial asthma of Abnormal Savda syndrome and Abnormal unsavda syndrome in Traditional Uyghur Medicine （TCM）.Methods Ser-um samples from 61 cases of Abnormal Savda syndrome bronchial asthma patients and 55 cases of Abnor-mal unsavda syndrome bronchial asthma patients are detected and described with 1 H-NMR based metabo-nomics,and than the data is analyzed by Orthogonal Partial Least Squares-Discriminant Analysis （OPLS-DA）.Results Comparing with the healthy people,the various kinds of amino acids of abnormal savda syndrome bronchial asthma patients are markedly lower,including isoleucine,leucine,alanine,valine, tyrosine,glycine （P〈0.05）.Glutamine,glucose,glycoprotein,creatine,lactic acid,carnitine,acetone, pyruvic acid,lipid （include LDL and VLDL）,unsaturated lipid are also markedly lower comparing with the health people （P〈0.05）.The another results show that the abnormal savda syndrome bronchial asth-ma patients have a higher degree of glucose,glycoprotein,lactamine,glutamic acid,valine,leucine,iso-leucine,glycine,tyrosine,phenylalanine,1-methylhistidine （P〈0.05）and a higher degree of acetoace-tate,acetone,pyruvic acid,lactate,creatine,creatinine,carnitine （P〈0.05）;another metabolites like lipid（include LDL and VLDL）,unsaturated lipid ,acetic acid,acetylcysteine,glutamine,scyllitol in plasma more than the unsavda syndrome bronchial asthma patients （P〈 0.05）.Conclusion Bronchial asthma patients of the abnormal savda syndrome had transformed in energy metabolism and protein metab-olism.It indicates that the abnormal gluconeogenesis and immunologic function disorder was more severe in bronchial asthma patients of the abnormal savda syndrome than in bronchial asthma patients of the unsavda syndrome.This may be one of the biological foundation from Abnormal Savda syndrome bronchial asthma forma-tion,also confirm the scientific basis of different syndromes in the same disease of Uygur Medicine.