不同针刺强度对急性脑缺血再灌注大鼠TNF-α、IL-1β含量的影响

目的探讨不同针刺强度对急性脑缺血再灌注大鼠肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)含量的影响。方法将44只SD雄性大鼠随机分成正常组(6只)、假手术组(8只)、模型组(30只),模型组又随机分为模型对照组、弱电针组、强电针组,每组8只,另6只造模备用。采用改良线栓法建立大鼠局灶性脑缺血(MCAO)再灌注模型,针刺"百会"、"大椎"穴,接电针仪:疏密波,频率2～15Hz,调整不同电流参数,强电针参数:以肢体震颤或肌肉出现轻微强直收缩为度,电流强度4～7mA;弱电针参数:以胡须微动或肌肉轻度跳动为度,电流强度1～3mA。24h后取材,用酶联免疫吸附试验(ELISA)检测各组血清中TNF-α、IL-1β含量并进行统计学分析。结果与正常组、假手术组比较,模型对照组TNF-α,IL-1β含量差异有统计学意义(P<0.01);与模型对照组比较,各电针组能明显降低模型大鼠血清TNF-α,IL-1β含量(P<0.01～0.05);与弱电针组比较,强电针组对TNF-α、IL-1β的调节作用显著(P<0.05)。结论不同针刺强度在调节急性脑缺血再灌注大鼠炎性细胞因子TNF-α、IL-1β含量有差异,强刺激优于弱刺激。

The influence of TNF-α and IL-1β by different electroacupuncture intensity on acute cerebral ischemia reperfusion in rats

Objective To explore the effects of different intensities of electroacupuncture to the contents of TNF-α and IL-1β on acute cerebral ischemia reperfusion in rats.Methods A total of 44 male SD rats were randomly divided into normal group(n=6),sham operation group(n=8),model group(n=30),model group was randomly divided into model compare group(n=8),weaker electroacupuncture group(n=8),stronger electroacupuncture group(n=8).Established the focal cerebral ischemia reperfusion model(MCAO) by modified suture method in rats,and given Electroacupuncture "Baihui","dazhui": sparse-density wave,frequency of 2~15 Hz,adjustment of different current parameters,strong Electroacupuncture Parameters: limb tremor or minor tonic contraction of muscle,and put current controled in 4~7 mA;weak Electroacupuncture parameters: beard fretting or muscle mild beating,and the current controled in 1~3 mA.Then sampling after 24 hours.TNF-α and IL-1β contents of rat serum were measured by enzyme linked immunosorbent assay(ELISA) and did the statistical analysis.Results Compared with the normal group and sham operation group,the contents of TNF-α and IL-1β in model compare group were significantly difference(P<0.01);compared with the model compare group,the contents of TNF-α and IL-1β were significantly decrease in the electroacupuncture group(P<0.05~0.01);Compared with the weaker electroacupuncture group,the regulation of TNF-α and IL-1β in the stronger electroacupuncture group was significant(P<0.05).Conclusion The therapeutic of different intensity electroacupuncture in the regulation of inflammatory cytokines TNF-α and IL-1β have differences in the rats of acute cerebral ischemia reperfusion.The therapeutic of strong stimulation is better than the weak stimulation.