经单宁酸处理脱细胞猪心脏瓣膜性能及形态学研究

目的：对比加单宁酸和传统的去氧胆酸钠法去除细胞后心脏瓣膜形态学及性能差别,为构建组织工程心脏瓣膜提供实验依据。方法：应用两种方法处理6～7月龄猪主动脉心脏瓣膜。光学显微镜、透射电镜观察脱细胞基质改变、DNA含量测定。结果：两种方法处理后,显微镜检和透射电镜见单宁酸-去氧胆酸钠法对基质破坏较少;两组均可以完全脱去瓣膜细胞。结论：经单宁酸处理能更好地保护去除瓣叶组织细胞外基质,提高其性能。

In vitro study on morphology and performance of cell extraction porcine cardiac valve treated by tannic acid

Objective：To compare the morphology and performance of the matrix after decellularization procedures with conventional sodium-deoxycholate method and the addition of tannic acid to the former method,in order to provide experimental basis for producing ideal tissue-engineered heart valves.Methods：Fresh aortic valves from porcine of 6-7 months were divided randomly into two groups.Valves in one group were treated with Triton-X100 and sodium deoxycholate,and those in the other group were treated with identical chemicals and tannic acid changes of the matrix structure and effects of treatment on the collagen and elastic fibers were observed by microscopy and transmission electron microscope and the levels of DNA content to confirm the removal of cells.Results：The heart valve leaflets were completely removed of the cell components by two methods.But the method of the addition of tannic acid produced little the harmfulness to the collagen and elastin of leaflets.Conclusion：Tannic acid can preserve the matrix structure efficiently during the decellularization procedure and improve the bio-mechanical properties and performance of tissue engineered cardiac valves.