AnA、AnuA、抗Sm抗体、抗dsDNA抗体测定在诊断SLE中的作用

目的：通过检测抗核抗体（AnA）、抗核小体抗体（AnuA）、抗Sm抗体、抗dsDNA抗体,探讨它们在系统性红斑狼疮（SLE）中的诊断价值。方法：检测120例SLE患者中的AnA、AnuA、抗Sm抗体、抗dsDNA抗体水平,AnuA采用ELISA法测定,抗Sm抗体采用免疫斑点法测定,抗dsDNA抗体及AnA采用间接荧光免疫法测定。结果：在检测的120例SLE患者中,AnA、AnuA、抗Sm抗体、抗dsDNA抗体的阳性率分别为97.5%、85.0%、42.5%、45.0%,在结缔组织中的阳性率分别为77.6%、1.0%、2.0%、3.0%。健康对照组中的阳性率分别为15.0%、0、0、1.0%。AnA、AnuA、抗Sm抗体、抗dsDNA抗体的检测对SLE患者的诊断特异性分别为22.4%、99.0%、98.0%、96.9%。结论：AnuA对SLE患者的诊断特异性最高;AnA对SLE诊断的阳性率最高,但其特异性最低,AnuA、抗Sm抗体、抗dsDNA抗体测定都可作为SLE诊断的可靠指标。

Roles of AnA,AnuA,anti-Sm antibody and anti-ds DNA antibody in the diagnosis of SLE

Objective：To explore the roles of antinuclear antibody（AnA）,anti-nucleosome antibody（AnuA）,anti-Sm an-tibody and anti-ds DNA antibody in the diagnosis of systemic lupus erythematosus（SLE）by detecting their levels.Meth-ods：The levels of AnA,AnuA,anti-Sm antibody and anti-ds DNA antibody in 120 patients with SLE were detected.AnuA was detected by using ELISA determination,anti-Sm antibody was detected by using immune dot determination,anti-ds DNA antibody and AnA were detected by using indirect fluorescent immunoassay.Results：In the detection of 120 patients with SLE,the positive rates of AnA,AnuA,anti-Sm antibody,anti-ds DNA antibody were 97.5%,85.0%,42.5%,45.0% respectively;the positive rates in connective tissue were 77.6%,1.0%,2.0%,3.0% respectively;the positive rates in healthy control group were 15.0%,0,0,1.0% respectively.The specificity of AnA,AnuA,anti-Sm antibody,anti-ds DNA antibody in the diagnosis of patients with SLE were 22.4%,99.0%,98.0%,96.9% respectively.Conclusion：AnuA shows the highest specificity for the diagnosis of SLE;AnA has a high positive rate in the diagnosis of SLE,but its specificity is the lowest,AnuA,anti-Sm antibody,anti-ds DNA antibody can serve as reliable indicators for the diagnosis of SLE.