肠易激综合征患者肠道菌群定量分析与意义

目的：了解不同类型肠易激综合征（IBS）患者和健康状况正常的人粪便中总厌氧菌群和需氧菌群及常见的4种厌氧菌和2种需氧菌的含量情况。方法：对临床上确诊的各类型IBS患者共52例和18例健康状况正常的志愿者，采集粪便标本进行总厌氧菌群和需氧菌群及常见的4种厌氧菌和2种需氧菌定量检测。结果：三种类型的IBS患者粪便中总厌氧菌群和需氧菌群数目与健康人差异明显，特别是双歧杆菌属和乳酸杆菌属明显减少（P〈0.01）；拟杆菌菌落数在腹泻型和混合型IBS患者中明显增加（P〈0.01）；肠球菌菌落数在腹泻型IBS患者中明显增加（P〈0.05）；需氧菌中患者组大肠杆菌和金黄色葡萄球菌菌落数均不同程度增加（P〈0.01或0.05），大肠杆菌菌落数在腹泻型IBS患者中增加最明显。结论：不同类型IBS患者肠道菌群紊乱表现不同，但均有双歧杆菌和乳酸菌属不同程度的减少，针对性的补充减少的益生菌可能是治疗IBS行之有效的方法。

Significance of intestinal tract normal bacteria flora quantitative analysis of IBS sufferers

Objective:To study the level of total anaerobic population,total aerobic population,four kinds of anaerobes and two kinds of aerobes in excrement of sufferers of varies kinds of irritable bowel syndrome(IBS) and that of normal persons.Methods:Excrement specimens from 52 IBS sufferers of different kinds and that of 18 normal volunteers were collected,and the level of four kinds of anaerobes and two kinds of aerobes were quantified.Results:The level of total anaerobic population and total aerobic population of excrement specimens of three kinds of IBS sufferers were different significantly,particularly the level of bifidobacterium and cillobacterium(P<0.01) ;the level of bacteroid population was higher significantly in diarrhea-predominant and mixed type of IBS sufferers(P<0.01) ;the level of enterococcus population was higher significantly in diarrhea-predominant IBS sufferers(P<0.05) ;among the aerobes,the number of Escherichia coli and Staphylococcus aureus colonies were increased by different level(P<0.01or0.05) in the IBS suffers,in particular,the number of Escherichia coli colonies in diarrhea-predominant IBS sufferers increased most significantly.Conclusion:Sufferers of different types of IBS have different intestinal tract dysbacteria phenotypes.But the level of bacillus bifidus and lactobacillus are always decreased by different degrees.Therefore,targeted supplement of probiotic bacteria which decrease in IBS sufferers would be an effective way of treatment.