低频脉冲磁场对兔膝关节软骨细胞人白介素1β、肿瘤坏死因子α表达与分泌的影响

目的探讨低频脉冲磁场对兔膝关节软骨细胞人白介素1β（IL-1β）、肿瘤坏死因子α（TNF-α）表达与分泌的影响。方法选择40只健康成年新西兰白兔建立兔膝关节软骨损伤模型,把右膝关节设为治疗组（n=20）,每日给予低频脉冲磁场治疗;左膝关节为对照组（n=20）,给予假磁场治疗,治疗后1、4周深麻醉处死动物,观察相关指标的变化。结果在同一观察时间点,IL-1β和TNF-α表达量治疗组均明显少于对照组,差异均有统计学意义（均P〈0.05）。治疗组软骨损伤程度较对照组轻微,关节活动度明显大于对照组,outerbridge积分也少于对照组,差异均有统计学意义（均P〈0.05）。结论低频脉冲磁场治疗骨性关节炎具有简便易行,安全无创等优点,可有效遏制IL-1β、TNF-α的释放,调节分泌作用,从而对骨性关节炎软骨损伤修复具有促进作用。

The effects of expression and secretion of human interleukin-1β and tumor necrosis factor α in rabbit articular cartilage cells by the low-frequency pulsed magnetic field

Objective To investigate the effects of expression and secretion of human interleukin-1 β(IL-1β),tumor necrosis factor α(TNF-α) in rabbit articular cartilage cells by the low-frequency pulsed magnetic field.Methods 40 healthy adult New Zealand white rabbits were selected to construct the articular cartilage injury model,the right knees were chosen as the treatment group(n=20) and given daily low-frequency pulsed magnetic field therapy;the left knees(n=20) were chosen as the control group and given false magnetic field therapy,after the treatment of 1 and 4 weeks,the rabbits were given deep anesthesia and sacrificed to observe the changes of the relevant indicators.Results At the same time points,the expression of IL-1β and TNF-α in the treatment group were lower than those in control group,the differences were all statistically significant(all P<0.05).The degree of cartilage damage in treatment group was less than that in control group,the outerbridge points in treatment group were less than that in control group,the joint activity of the treatment group were better than that of the control group,the differences were all statistically significant(all P<0.05).Conclusion The low-frequency pulse magnetic field therapy for treating osteoarthritis is simple,safe and non-invasive which can effectively curb the release and regulate the secretion of IL-1β and TNF-α,thus it can promote the repair of the osteoarthritis cartilage injury.