HPLC法测定银翘解毒颗粒中连翘苷和牛蒡子苷的含量

目的：建立以RP-HPLC法测定银翘解毒颗粒中连翘苷和牛蒡子苷含量的方法。方法：采用Agilent C18（4．6mm×250mm，5μm）色谱柱，流动相：乙腈-水（27：73），检测波长：280nm，柱温：30℃，流速：1．0ml／min。结果：连翘苷在0．02142～0．10710μg范围内进样量与峰面积呈良好线性关系，平均加样回收率为99．93％，RSD为0．71％；牛蒡子苷在0．8368-6．6940μg范围内进样量与峰面积呈良好线性关系，平均加样回收率为99．75％，RSD为0．44％。3批样品中连翘苷和牛蒡子苷的含量分别为0．04318、1．07300mg]g；0．04306、1．07000mg／g；0．04310、1．07100mg／g。结论：本法灵敏、准确，专属性强，重现性好，可作为银翘解毒颗粒中连翘苷和牛蒡子苷的含量测定方法。

Simultaneous determination of Arctiin and Forsythin in Yingqiao Jiedu Granules by HPLC

Objective： To establish a method for determination of arctiin and forsythin in Yingqiao Jiedu Granules by HPLC. Methods： The analytical column Cj8 （4.6 mm×250mm,5μm） was used. The mobile phase was composed of acetonitrile and water （27：73） at a flow rate of 1.0 ml/min. The wavelength of UV detector was 280 nm and column temperature was 30℃. Results： The linear ranges of arctiin was 0.021 42-0.107 10 μg. The average recovery was 99.93% with a RSD of 0.71%. The linear ranges of forsythin was 0.836 8-6.694 0 μg. The average recovery was 99.75% with a RSD of 0.44%. The content of arctiin and forsythin in three batches of samples were 0.043 18, 1.073 00 mg/g; 0.043 06, 1.070 00 mg/g; 0.043 10, 1.071 00 mg/g. Conclusion： This method is sensitive, accerate, exclusive reproducible. It can be applied to determinate arctiin and forsythin in Yingqiao Jiedu Granules.