肝硬化患者血清中基质金属蛋白酶-3、 基质金属蛋白组织抑制因子-1 水平与肝硬化进程的研 究简

目的 探讨肝硬化患者血清中的基质金属蛋白酶-3（MMP-3）和基质金属蛋白组织抑制因子-1 （TIMP-1）值对于肝硬化进程的影响.方法 分析台州恩泽医疗集团路桥医院2011年1月～2012年6月收治的30例乙型肝炎后肝硬化代偿期患者（代偿组）及30例失代偿期患者（失代偿组）的临床资料,30名健康者作为正常对照组.采用BAS-ELISA法测定MMP-3的血清含量,同时采用ELISA双抗体夹心法测定TIMP-3的浓度;计算TIMP-1/MMP-3的比值.对比两组患者的MMP-3、TIMP-1的临床指标的异同.结果 ①代偿组患者血清MMP-3水平（20.32±3.10） ng/mL]显著高于正常对照组血清MMP-3水平（17.45±2.30）ng/mL],差异有统计学意义（P＜ 0.05）;代偿组患者血清TIMP-1水平（1.72±0.40）ng/mL]显著高于正常对照组（1.49±0.30）ng/mL],差异有统计学意义（P＜0.05）;代偿组TIMP-1/MMP-3值为（0.088±0.010）,比正常对照组TIMP-1/MMP-3值（0.086±0.010）有所升高,但差异无统计学意义（P＞0.05）.②失代偿组患者血清MMP-3水平（22.73±5.20）ng/mL]显著高于正常对照组（17.45±2.30）ng/mL],差异有统计学意义（P＜0.05）;失代偿组患者血清TIMP-1水平（2.24±0.30）ng/mL]显著高于正常对照组（1.49±0.30）ng/mL],差异有统计学意义（P＜0.05）;失代偿组患者TIMP-1/MMP-3值为（0.103±0.020）,显著高于正常对照组（0.086±0.010）,差异有统计学意义（P＜0.05）.③失代偿组患者血清中MMP-3水平显著高于代偿组患者,差异有统计学意义（P＜0.05）;失代偿组患者血清TIMP-1水平显著高于代偿组患者,差异有统计学意义（P＜0.05）;失代偿组患者TIMP-1/MMP-3值显著高于代偿组患者,差异有统计学意义（P＜ 0.05）.结论 代偿期肝硬化患者MMP-3以及TIMP-1皆显著升高,TIMP-1/MMP-3比值有升高趋势.失代偿期肝硬化患者MMP-3以及TIMP-1皆显著升高,TIMP-1/MMP-3比值显著升高.MMP-3/TIMP-1的比值与TIMP-1的表达与肝硬化程度有关.

Study of serum MMP-3, TIMP-1 levels in liver cirrhosis process

Objective To study the influence of serum MMP-3,TIMP-1 levels in liver cirrhosis process.Methods 60 patients,who were treated in Luqiao Hospital of Taizhou Enze Medical Group from January 2011 to June 2012 were selected.The clinical data of 30 patients with hepatitis B and compensating liver cirrhosis were chosen as compensating group,30 patients with decompensating liver cirrhosis were selected as decompensating group,and 30 healthy people were seen as normal control group.BAS-ELISA was established to determine the concentration of MMP-3 in serum,and double antibody sandwich ELISA was established to determine of the concentration of TIMP-3 and calculate the ratio of TIMP-1/MMP-3.The clinical indicators of MMP-3,TIMP-1 in two groups of patients were compared to find the similarities and differences.Results ①MMP-3 and TIMP-1 level in serum of compensating group （20.32± 3.10）,（1.72±0.40） ng/mL] were higher than those of normal control group （17.45±2.30）,（1.49±0.30） ng/mL],the differences were statistically significant （P 〈 0.05）; the value of TIMP-1/MMP-3 in compensating group （0.088±0.010） were little higher than that in normal control group （0.086±0.010）,but the difference was not statistically significant （P 〉 0.05）.②MMP-3 level,TIMP-1 level and the value of TIMP-1/MMP-3 in serum of decompensating group （22.73± 5.20）,（2.24±0.30） ng/mL,（0.103±0.020）] were all higher than those of normal control group （17.45±2.30）,（1.49±0.30）ng/mL,（0.086±0.010）],the differences were statistically significant （P 〈 0.05）.③MMP-3 level in serum of decompensating group was higher than that in compensating group,the difference was statistically significant （P 〈 0.05）; TIMP-1 level in serum of decompensating group was higher than that in compensating group,the difference was statistically significant （P 〈 0.05）; TIMP-1/MMP-3 value in decompensating group was higher than that in compensating group,the difference was statistically significant （P 〈 0.05）.Conclusion MMP-3 level and TIMP-1 level of compensating liver cirrhosis patients are significantly increased,and there is a trend that the ratio of TIMP-1/MMP-3 will increase.MMP-3 level and TIMP-1 level of decompensating liver cirrhosis patients are significantly increased,the value of MMP-3/TIMP-1 is significantly increased.There is a relationship between the ratio of MMP-3/TIMP-1,the expression of TIMP-1 and the extent of liver cirrhosis.