反相高效液相蒸发光散射检测法测定三金软胶囊中羟基积雪草苷含量

目的：建立测定三金软胶囊中羟基积雪草苷含量的方法。方法：采用反相高效液相色谱法测定羟基积雪草苷含量。色谱条件为DiamonsilC18柱（150mm×4．6mm，5μm），流动相为甲醇-水（50：50），检测器为蒸发光散射检测器。结果：羟基积雪草苷在1．006～10．064雌的范围内，进样量与峰面积呈良好的线性关系（r=0．9992），精密度、8h内稳定性、重复性均符合要求，平均回收率为100．16％（RSD=2．28％）。结论：该含量测定方法灵敏、准确，重复性好，专属性强，适合羟基积雪草苷的含量测定。

Determination of madecassoside in Sanjin Soft Capsulae by RP-HPLC-ELSD

Objective：To determine the content of madeeassoside in Sanjin Soft Capsulae. Methods：HPLC method was used to determine the content of madecassoside. The column was Diamonsil C18 （150 mm×4.6 mm,5 μm）,the mobile phase was methanol-water （50：50）,and the evaporative light-scattering detector （ESLD） was used as detector. Results：The linear range was 1.006-10.064 μg （r=0.999 2）, precision, stability and reproducibility were good, the average recovery was 100.16% （RSD=2.28%）. Conclusion ：The method is sensitive, accurate with good reproducibility and strong specificity, and it can be used for the determination of madecassoside.