焦虑大鼠模型高架十字迷宫实验的复测信度检验及参数相关性分析

目的：探讨高架十字迷宫（elevated plus-maze,EPM）作为焦虑大鼠模型评价方法的复测信度及其参数相关性。方法：将成年雌性Wistar大鼠放入高架十字迷宫中,摄像系统记录5 min的行为变化,实验间隔1周进行2次;实验参数如下：进入开放臂次数、进入封闭臂次数、向下探究次数、封闭臂后腿直立次数、进入开放臂时间、进入封闭臂时间。结果：①组内相关系数（ICC）结果表明,除封闭臂后腿直立次数和向下探究次数初测与复测相关性差外,其他参数均显示EPM的初测与复测具有较好的相关性。②一致性系数（Kappa）结果表明,所有参数均显示EPM复测可信度较好,Kappa值均〉0.5。结论：进入开放壁时间百分比、进入开放壁次数百分比和总进臂次数在EPM初测及复测实验中均呈较好复测信度。

Retest reliability test and parameter correlation analysis of elevated plus-maze experiment for anxious rat model

Objective:To investigate the retest reliability and parameter correlation of the elevated plus-maze(EPM) experiment as the evaluation method of anxious rat model.Methods:The camera system recorded the behavioral changes within 5 minutes of adult female Wistar rats placed in the elevated plus-maze twice every other week.The experimental parameters were as follows:times of entering the open arm,times of entering the closed arm,times of downward exploration,times of rear leg upright standing in the closed arm,...