| HPLC法测定氨茶碱注射液中茶碱的含量 |
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| 引用本文： | 王玉红,郑凯.HPLC法测定氨茶碱注射液中茶碱的含量[J].中国医药导报,2010,7(19):71-72. |
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| 作者姓名： | 王玉红 郑凯 |
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| 作者单位： | 辽宁省朝阳市药品检验所,辽宁,朝阳,122000 |
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| 摘 要： | 目的：建立HPLC法测定氨茶碱注射液中茶碱的含量。方法：采用Kromonsil C18（4.6mm×150mm,5μm）色谱柱;流动相：甲醇-0.01mol/LKH2PO4（30：70）（三乙胺调节pH=6.5）;流速：1.0ml/min;检测波长：271nm;结果：茶碱在9.98～199.68μg/ml范围内线性关系良好（r=0.9999）,高、中、低3种浓度平均加样回收率为98.93%,RSD=1.47%（n=9）。结论：本法简便、准确,分离效果佳,可用于测定氨茶碱注射液中茶碱的含量。
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| 关 键 词： | HPLC 氨茶碱注射液 茶碱 |
Content determination of Theophylline in Aminophylline Injection by HPLC |
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| WANG Yuhong,ZHENG Kai.Content determination of Theophylline in Aminophylline Injection by HPLC[J].China Medical Herald,2010,7(19):71-72. |
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| Authors: | WANG Yuhong ZHENG Kai |
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| Institution: | (Chaoyang Institute for Drug Control,Liaoning Province,Chaoyang 122000,China) |
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| Abstract: | Objective：To establish a method for the determination of theophylline in Aminophylline Injection by HPLC.Methods：Kromonsil C18 column（4.6 mm×150 mm,5 μm） was used.The mobile phase consisted of a mixture of methanol and 0.01 mol/L-KH2PO4（30：70）（adjusted pH to pH=6.5 with triethylamine）.The mobile rate was 1.0 ml/min.The detection wavelength was at 271 nm.Results：The linear range was 9.98-199.68 μg/ml（r=0.999 9）.The average recovery was 98.93% with a RSD of 1.47%（n=9）.Conclusion：The method is simple and accurate,it can be used for the determimnation of Theophylline in Aminophylline Injection. |
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| Keywords: | HPLC |
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