| 重组人肝细胞生长因子基因的克隆 |
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| 引用本文： | 罗建新 谭文斌. 重组人肝细胞生长因子基因的克隆[J]. 湖南医科大学学报, 2000, 25(5): 425-427 |
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| 作者姓名： | 罗建新 谭文斌 |
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| 摘 要： | 用ＲＴ－ＰＣＲ技术从人胎盘组织内成功地扩增全长肝细胞生长因子（ＨＧＦ）ｃＤＮＡ基因（２ １８４ｂｐ），并将其克隆至ｐＧＥＭ－Ｔ载体，经限制性核酸内切酶ＮｄｅⅠ，ＢｇｌⅡ，ＨｉｎｄⅢ，ＢａｍＨⅠ和ＸｈｏⅠ的酶谱分析和ＤＮＡ测序分析证实。再将其亚克隆至逆病毒载体ｐＬＮＬ－ＸＨＣ，可进一步用于基因表达和基因治疗的研究。
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| 关 键 词： | 肝细胞生长因子 基因克隆 逆病毒载体 |
Cloning of the recombinant human hepatocyte growth factor gene] |
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| J X Luo,W B Tan,Y N Tan,G J Chen. Cloning of the recombinant human hepatocyte growth factor gene][J]. Bulletin of Hunan Medical University, 2000, 25(5): 425-427 |
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| Authors: | J X Luo W B Tan Y N Tan G J Chen |
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| Affiliation: | Department of Clinical Biochemistry, Research Center of Molecular Biology, Hunan Medical University, Changsha 410078. |
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| Abstract: | The full length of HGF cDNA gene(2,184 bp) was amplified successfully from human placental tissue using RT-PCR technique, and then cloned into pGEM-T vector, which was identified and confirmed by restriction endonuclease mapping using Nde I, Bg1 II, Hind III, BamH I and Xho I, as well as DNA sequencing. The above clone of HGF cDNA gene was successfully subcloned into the retrovirus vector(pLNL-XHC), which may be used for further studies of gene expression and gene therapy. |
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