| 血小板衍生生长因子和转化生长因子—β1在致敏大鼠模型肺泡巨噬细胞中的表达变化 |
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| 引用本文： | 潘频华,陈清兰,等.血小板衍生生长因子和转化生长因子—β1在致敏大鼠模型肺泡巨噬细胞中的表达变化[J].湖南医科大学学报,2001,26(4):328-330. |
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| 作者姓名： | 潘频华 陈清兰 |
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| 摘 要： | 目的：探讨肺泡巨噬细胞在支气管哮喘气道重塑中的作用。方法：16只Wistar大鼠随机分成2组：正常组、哮喘模型组，每组8只，采用鸡卵清白蛋白腹腔致敏和反复雾化吸入复制大鼠过敏性哮喘气道炎症模型。应用免疫组化和原位杂交法观察血小板衍生生长因子－b(PDGF-b)、转化生长因子－β1（TGF－β1）在肺泡巨噬细胞中的表达。结果：2组间肺泡巨噬细胞PDGF－b蛋白表达阳性率和表达强度差异无显著性，而模型组TGF－β1蛋白表达阳性率（P＜0．05）和表达强度计分（P＜0．05）高于正常组。原位杂交法显示，模型组TGF－β1 mRNA的表达阳性率（P＜0．05）亦高于正常组。结论：哮喘肺泡巨噬细胞表达高水平TGF－β1可能参与哮喘气道重塑过程。
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| 关 键 词： | 肺泡巨噬细胞 气道重塑 哮喘 动物疾病模型 血小板衍生生长因子 转化生长因子β1 |
Expression of platelet-derived growth factor-b and transforming growth factor-beta 1 in the alveolar macrophages of allergic rats] |
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| P H Pan,Q L Chen,E S Wu.Expression of platelet-derived growth factor-b and transforming growth factor-beta 1 in the alveolar macrophages of allergic rats][J].Bulletin of Hunan Medical University,2001,26(4):328-330. |
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| Authors: | P H Pan Q L Chen E S Wu |
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| Institution: | Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha 410008, China. |
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| Abstract: | OBJECTIVE: To investigate the role of alveolar macrophages in airway remodeling in asthma. METHOD: Sixteen Wistar rats were divided into two groups: control group and asthmatic group. The expressions of platelet-derived growth factor-b (PDGF-b) and transforming growth factor-beta. (TGF-beta 1) in the alveolar macrophages of allergic rats were detected by immunohistochemistry and in situ hybridization. RESULTS: The expression of TGF-beta 1 in alveolar macrophages in allergic group was increased in both protein level (P < 0.05) and TGF-beta 1 mRNA(P < 0.01); but the expression of PDGF-b had no difference between the two groups. CONCLUSION: The alveolar macrophages may involve in the airway remodeling of asthma. |
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