载脂蛋白E基因对血脂代谢的调节及其与冠心病的关系

应用改良的聚合酶链反应－限制性片断长度多态性的方法，测定１０２例正常人和１１８例冠心病患者的载脂蛋白（Ａｐｏ）Ｅ基因型；并分析该基因对两组受试者的血脂、脂蛋白和Ａｐｏ水平的影响。结果显示，ＡｐｏＥ４等位基因具有升高血浆总胆固醇（ＴＣ）、低密度脂蛋白胆固醇（ＬＤＬ－Ｃ）和ＡｐｏＢ１００的作用；而ＡｐｏＥ２等位基因则有相反的作用。冠心病组ＡｐｏＥ４等位基因频率显著高于对照组。提示ＡｐｏＥ基因对血脂水平

Effect of apolipoprotein E gene plasma levels of lipids, lipoproteins, apolipoproteins and its relation with coronary heart disease]

Using a technique of polymerase chain reaction-restriction lengths polymorphism (PCR-RLP), the authors detected the genotypes of apolipoprotein EE(ApoE) in 102 normal control subjects and in 118 patients with coronary heart disease (CHD). Meanwhile, the effect of ApoE allele on plasma lipid, lipoprotein, apolipoprotein levels were analyzed, and ApoE allele frequencies of patients with CHD and of control subjects were compared. The results showed that the technique of modified PCR-RFLP was a useful measure to detect the genotypes of ApoE. ApoE gene was polymorphism, resulting in plasma lipid level variance among individuals. As compared to ApoE3 allele, ApoE4 allele had positive effect on plasma TC, LDL-C and ApoB100 levels; while ApoE2 allele had negative effect on plasma TC, LDL-C and ApoB100 levels. The frequency of E4 allele in patients with CHD was higher than that in control subjects. The results in the present study suggest that ApoE4 allele is probably the genetic marker of CHD.