转染人乳头瘤病毒的宫颈癌U14移植同系小鼠后的生长特征和转移规律

Growth behavior and metastatic pattern of cervical cancer U14 transfected with human papillomavirus in inbred mouse]

OBJECTIVE: To investigate the growth behavior and metastatic pattern of murine cervix cancer U14 transfected with human papillomavirus(HPV) in inbred 615-strain mouse. METHODS: We transfected HPV 16 E6 and E7 genes into mouse cervix carcinoma cell strain NO. 14(U14) by electroporation and liposome, respectively. The transfectants were selected by G418, and several high-expressed HPV16 E6 and E7 clonal cell lines (E6+ U14, E7+ U14) were detected by PCR and by immunohistochemistry. We transplanted those cells into inbred 615-strain mice both subcutaneously and intraperitoneally to observe the growth behavior and metastasis of them. RESULTS: The durations of tumor appearance were 5-7 d, 11-14 d, and 8-10 d after having been transplanted subcutaneously with wild type U14, E6+ U14, and E7+ U14, respectively(P < 0.05). The mean survival times of mice were 29 d, 43 d, and 35 d, respectively. Metastasis could be found both in lymph nodes (90%, 30%, and 40%, respectively) and lungs (60%, 10%, and 20%, respectively). After intraperitoneal inoculation, the mean survival durations of mice were 14.2 d, 20.6 d and 18.3 d. We could not find metastasis both in lymph nodes and lungs. CONCLUSION: Murine cervix cancer U14 cells transfected with HPV16 E6 and E7 have different growth behavior and metastatic patterns after transplanted in inbred mice, which provide useful models for studying their immunotherapy or other strategies for cervical cancer with E6 and E7 as a target.