乳香提取物诱导Jurkat细胞凋亡的实验研究

检测不同浓度、不同时间作用了下乳香提取物对人急性Ｔ淋巴细胞血病细胞株Ｊｕｒｋａｔ细胞的促凋亡作用。方法：用琼脂糖凝胶电泳技术、透射电子显微镜（ＴＥＭ）和流式细胞仪（ＦＣＭ）分别检测Ｊｕｒｋａｔ细胞的ＤＮＡ梯状带，形态学改变及凋亡峰与药物作用的细胞周期。

Experimental study on Jurkat cell apoptosis induced by Boswellia carterii Birdw extractive]

OBJECTIVE: To study the influence of Boswellia carterii Birdw(BCB) extractive of different concentrations on human Jurkat cell apoptosis at different time points. METHODS: Agarose gel electrophoresis, transmission electron microscope(TEM) and flow cytometry(FCM) were used for observing DNA ladders, morphology of Jurkat cells and cell cycle, respectively. RESULTS: BCB induced apoptosis of Jurkat cells and typical DNA ladders; TEM demonstrated the presence of apoptosis Jurkat cells, condensation of cytoplasm, numerous vaculoes in cytoplasm, compaction of the nuclear chromatin and formation of apoptosis dody. The sub-G1 peak was detected by cell cycle analysis. It revealed that G1 phage cells increased and S phage cells decreased. CONCLUSION: The data indicate that BCB extractive induces time- and concentration-dependent apoptosis in Jurkat cell line.