脑溢安颗粒对脑出血大鼠脑组织bcl—2表达的影响

目的：探讨脑安颗粒（简称脑溢安）治疗脑出血的疗效机制。方法：立体定位于苍白球内注射Ⅶ型胶原酶诱导大鼠脑出血模型，舸用免疫组织化学法检测脑溢安组与各对照组脑组织内bcl-2蛋白表达。结果：模型组脑组织内bcl-2阳性细胞在1d开始增加，2d达峰值，7d时明显减少；脑溢安组其bcl-2阳性细胞在2d，4d，7d明显高于模型组。结论：脑溢安可上调bcl-2表达。

Effects of nao-yi-an granule on bcl-2 expression in the rat brain after experimental cerebral hemorrhage]

OBJECTIVE: To investigate the protective effects and mechanisms of the traditional Chinese medicine complex, nao-yi-an granule (NYA) on experimental intracerebral hemorrhage (ICH) in rats. METHODS: ICH models anesthesized were induced by stereotaxical injection of 2 microliters normal saline containing 0.4 U bacterial collagenase(type VII) into the right globas pallidus(GP), and the protein expression of bcl-2 in the rat brain after ICH was detected with the immunohistochemistry method, with which the effects of NYA were observed. RESULTS: Bcl-2 positive cells were found in the cerebral cortex and basal ganglia area after ICH. Immunoreactivity was localized in the cytoplasm as a fine granular precipitate. The levels of bcl-2 expression in the model group varied at different time-points, and reached the peak at 2 d. In the NYA group, the protein expression of bcl-2 was increased significantly both in the cortex and in the basal ganglia compared with those in the model group. CONCLUSION: NYA can significantly upregulate the protein expression of bcl-2.