爱西特对小分子尿毒素的吸附作用及其影响因素

目的：研究爱西特对小分子尿毒素的吸附能力及影响其吸附的因素。方法：将尿素、肌酐、尿酸标准品加入蒸馏水配成尿毒素溶液并加入爱西特作用不同时间，检测其尿毒素成份。干扰组分别加胆汁、氨基酸、林格氏液、葡萄糖液、Hcl和NaOH，并同时加入爱西特吸附后检测尿毒素浓度变化。结果：尿素、肌酐、尿酸浓度5min开始下降，尿素40min达最低值，50min后有反弹，肌酐、尿酸10～30min达最低值，以后趋稳定。胆汁、氨基酸、电解质、葡萄糖液、酸碱等对爱西特吸附尿酸无明显影响，但对尿素、肌酐吸附有不同程度减弱作用，胆汁、氨基酸对尿素干扰最大，葡萄糖液、酸碱次之；pH2．0液对肌酐吸附干扰最大，葡萄糖次之。结论：爱西特对尿素、肌酐、尿酸有良好的吸附能力．但随著吸附时间的延长．吸附能力下降。胆汁、含氮物、糖及不同DH对爱西特的吸附有不同程度的影响。

Adsorbent effect of activated carbon on small molecular uremic toxin and its influence factors]

OBJECTIVE: To analyze the adsorbent effect of activated carbon on uremic toxin and its influence factors. METHODS: Uremic toxins (urea, creatinine and uric acid) were dissolved in the distilled water to obtain uremic toxic solution. Activated carbon was added to the solution, and the concentrations of uremic toxins were measured at different time spots. To determine the influence factors, some possible related materials, such as bile, amino acid, Ringer's, solution of glucose, HCl or NaOH respectively were added simultaneously. RESULTS: The concentrations of toxins in uremic toxic solution decreased 5 min after adding the activated carbon. The concentration of urea was the lowest at 30 min, but it increased after 50 min; while the concentrations of creatinine and uric acid reached the lowest level from 10 to 30 min after adding the activated carbon, and maintained at the same level after that. The bile, amino acid, electrolyte, glucose and pH value did not influence the adsorption of uric acid significantly, but they influenced the adsorption of urea and creatinine. Bile and amino acid influenced the concentration of urea remarkably, following glucose, NaOH and HCl. The effect of pH 2.0 solution on the creatinine concentration was the most significant, following glucose. CONCLUSION: Activated carbon has adsorptive effect on uremic toxins, but its adsorptive effect decreases as time goes on. Bile, glucose, amino acid, NaOH and HCl can affect the adsorptive effect of activated carbon on uremic toxins to some extent.