| Physico-chemical Characterization of the Lipopolysaccharide (Endotoxin) Isolated from Bacteriodes fragilis NCTC 9343 |
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| 作者姓名： | 焦炳华 何炬斌 余庆 赵虎 |
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| 作者单位： | Department of Microbiology Second Military Medical University Shanghai，Department of Microbiology Second Military Medical University Shanghai，Department of Microbiology Second Military Medical University Shanghai，Department of Experimental Medicine Changzheng Hospital Second Military Medical University Shanghai |
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| 摘 要： | The lipopolysaccharide (LPS, endotoxin) isolated from Bacteriodes fragilis NCTC 9343 ex-hibted an endogenous heterogencity consisting of R (rough) and SR (semi-rough ) LPS fractionsupon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The LPS was chemically consti-tuted by D-glucosamine, D-glucose. L-rhamnose, D-galactose. fatty acids and phosphate in amolar ratio of 2: 1: 1: 4: 5. 4.: 2.7 The fatty acids were predominated by 3-hydroxypentadecanoic(3-OH-15: 0), 3-hydroxyhcxadecanoic (3-OH-16: 0), 3-hydroxyheptadecanoic (3-OH-17: 0), 3-hydroxy-15-methyl hexadecanoic (3-OH-15-Me-16: 0) and 13-methyl-letradecanoic acid (13-Mc-14:0). Approximately 1 mol of 2-Keto-3-deoxy-D-mannooctonatc (KDO) was detected after treat-ment of LPS with 48% of hydrofluoric acid (HF), indicating it was present as a phosphorylatedmolecule.
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Physico-chemical Characterization of the Lipopolysaccharide (Endotoxin) Isolated from Bacteriodes fragilis NCTC 9343 |
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| Jiao Binghua,He Jubin,Yu Qing.Physico-chemical Characterization of the Lipopolysaccharide (Endotoxin) Isolated from Bacteriodes fragilis NCTC 9343[J].Journal of Medical Colleges of PLA(China),1989(1). |
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| Authors: | Jiao Binghua He Jubin Yu Qing |
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| Institution: | Jiao Binghua,He Jubin,Yu Qing,Department of Microbiology,Second Military Medical University,ShanghaiZhao Hu,Department of Experimental Medicine,Changzheng Hospital,Second Military Medical University,Shanghai |
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| Abstract: | The lipopolysaccharide (LPS, endotoxin) isolated from Bacteriodes fragilis NCTC 9343 ex- hibted an endogenous heterogencity consisting of R (rough) and SR (semi-rough ) LPS fractions upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The LPS was chemically consti- tuted by D-glucosamine, D-glucose. L-rhamnose, D-galactose. fatty acids and phosphate in a molar ratio of 2: 1: 1: 4: 5. 4.: 2.7 The fatty acids were predominated by 3-hydroxypentadecanoic(3- OH-15: 0), 3-hydroxyhcxadecanoic (3-OH-16: 0), 3-hydroxyheptadecanoic (3-OH-17: 0), 3- hydroxy-15-methyl hexadecanoic (3-OH-15-Me-16: 0) and 13-methyl-letradecanoic acid (13-Mc-14: 0). Approximately 1 mol of 2-Keto-3-deoxy-D-mannooctonatc (KDO) was detected after treat- ment of LPS with 48% of hydrofluoric acid (HF), indicating it was present as a phosphorylated molecule. |
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| Keywords: | Bacteriodes fragilis lipopolysaccharide (cudotoxin) physical analysis chemical analysis |
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