| Effects of herpes simplex virus thymidine kinase/acyclovir system on growth of human pulmonary adenocarcinoma A549 cell line in vitro and in vivo |
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| 引用本文： | 何祥梁,何东华,郭先健,钱桂生,黄桂君,陈维忠,李淑萍. Effects of herpes simplex virus thymidine kinase/acyclovir system on growth of human pulmonary adenocarcinoma A549 cell line in vitro and in vivo[J]. 中国人民解放军军医大学学报, 2002, 17(3): 227-231 |
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| 作者姓名： | 何祥梁 何东华 郭先健 钱桂生 黄桂君 陈维忠 李淑萍 |
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| 作者单位： | Department of Respiratory Diseases，Zhujiang Hospital，First Military Medical University，Guangzhou 510282，China，Da'an Gene Center，College of Medical Sciences，Sun Yat-sen University，Guangzhou 510120，China，Institute of Respiratory Diseases of PLA，Xinqiao Hospital，Third Military Medical University，Chongqin 400037，China，Institute of Respiratory Diseases of PLA，Xinqiao Hospital，Third Military Medical University，Chongqin 400037，China，Institute of Respiratory Diseases of PLA，Xinqiao Hospital，Third Military Medical University，Chongqin 400037，China，Institute of Respiratory Diseases of PLA，Xinqiao Hospital，Third Military Medical University，Chongqin 400037，China，Institute of Respiratory Diseases of PLA，Xinqiao Hospital，Third Military Medical University，Chongqin 400037，China |
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| 摘 要： | Objective: To observe the effect of anciclovir (ACV) treatment on tumors induced by inoculation of TK gene-transfected human pulmonary adenocarcinoma A549 cells in nude mice. Methods: A recombinant plasmid containing TK gene was constructed and transfected into A549 cells by electroporation. The sensitivity of the transgenic cells (A549-TK) to ACV was examined by MTT assay in vitro and for in vivo observation, inoculation of A549-TK and A-549 cells into nude mice was separately performed to induce tumor growth, the response of which to ACV treatment was observed, and the tumor tissues were pathologically examined. Results: A recombinant plasmid containing TK gene was successfully constructed and transfected into A549 cells. The sensitivity of A549-TK cells to ACV was 43 times higher than that of A549 cells. The tumors induced by A549-TK cells showed no significant increase in size after ACV treatment (P>0. 05) , and light microscopy revealed local tissue necrosis, karyoklasis, and nuclei disappearance.
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| 关 键 词： | 单纯疱疹病毒胸苷激酶 阿昔洛韦系统 人肺腺癌细胞系 A549 体内生长 体外生长 基因治疗 |
Effects of herpes simplex virus thymidine kinase/acyclovir system on growth of human pulmonary adenocarcinoma A549 cell line in vitro and in vivo |
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| HE Xiang-liang,HE Dong-hua,GUO Xian-jian,QIAN Gui-sheng,HUANG Gui-jun,CHEN Wei-zhong,LI Shu-ping. Effects of herpes simplex virus thymidine kinase/acyclovir system on growth of human pulmonary adenocarcinoma A549 cell line in vitro and in vivo[J]. Journal of Medical Colleges of PLA(China), 2002, 17(3): 227-231 |
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| Authors: | HE Xiang-liang HE Dong-hua GUO Xian-jian QIAN Gui-sheng HUANG Gui-jun CHEN Wei-zhong LI Shu-ping |
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| Abstract: | Objective: To observe the effect of anciclovir (ACV) treatment on tumors induced by inoculation of TK gene-transfected human pulmonary adenocarcinoma A549 cells in nude mice. Methods: A recombinant plasmid containing TK gene was constructed and transfected into A549 cells by electroporation. The sensitivity of the transgenic cells (A549-TK) to ACV was examined by MTT assay in vitro and for in vivo observation, inoculation of A549-TK and A-549 cells into nude mice was separately performed to induce tumor growth, the response of which to ACV treatment was observed, and the tumor tissues were pathologically examined. Results: A recombinant plasmid containing TK gene was successfully constructed and transfected into A549 cells. The sensitivity of A549-TK cells to ACV was 43 times higher than that of A549 cells. The tumors induced by A549-TK cells showed no significant increase in size after ACV treatment (P>0. 05) , and light microscopy revealed local tissue necrosis, karyoklasis, and nuclei disappearance. Conclusion: A549-TK cells acquires sensitivity to ACV both in vitro and in vivo, and ACV can inhibit the growth of tumors induced by A549-TK cell inoculation in nude mice. |
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| Keywords: | pulmonary carcinoma herpes simplex virus thymine kinase gene gene therapy acyclovir nude mice |
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