| Differentially expressed phosphoproteins in diazoxide-pretreated ventricular myocytes by two-dimensional electrophoresis and mass spectrometry in vitro |
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| 作者姓名： | 李洪 肖颖彬 高玉琪 杨天德 |
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| 作者单位： | [1]Departments of Anesthesiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037,China [2]Departments of Cardiovascular Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing400037, China [3]Department of Pathophysiology, College of High Altitude Military Medicine, Third Military Medical University, Chongqing 400038, China |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | Objectives To analyze and identify differentially expressed phosphoproteins associated with mitochondrial KATP channel opening. Methods： Adult rat ventricular myocytes were isolated, cultured, and identified, and pretreated without or with 100 μmol/L diazoxide for 10 min. Phosphoproteins prepared and enriched from the control and diazoxide-pretreated cells were separated by two-dimensional gel electrophoresis （2-DE） followed by sliver staining. The obtained interesting phosphoproteins were further identified by mass spectrometry. Results. Associated with diazoxide preconditioning, the proteins of chaperonin containing TCP-1 and hypothetical protein XP-346548 were phosphorylated significantly （P〈0. 01）, while the 94-kDa glucose-regulated protein, calpactin I heavy chain and ferritin were dephosphorylated markedly （P〈0. 01）. Conclusion： These findings suggest that cardiomyocytes undergo significant posttranslational modification via phosphorylation in a multitude of proteins in order to respond diazoxide preconditioning, and these phosphorylated protein may mediate the downstream signaling of cardioprotection by mitochondrial KATp channel opening induced by ischemic preconditioning.
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| 关 键 词： | 磷蛋白质 氯甲苯噻嗪 心肌细胞 电泳实验 |
| 收稿时间： | 2005-09-28 |
| 修稿时间： | 2006-01-15 |
Differentially expressed phosphoproteins in diazoxide-pretreated ventricular myocytes by two-dimensional electrophoresis and mass spectrometry in vitro |
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| LI Hong,XIAO Ying-bin,GAO Yu-qi,YANG Tian-de.Differentially expressed phosphoproteins in diazoxide-pretreated ventricular myocytes by two-dimensional electrophoresis and mass spectrometry in vitro[J].Journal of Medical Colleges of PLA(China),2006,21(3):143-147. |
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| Authors: | LI Hong XIAO Ying-bin GAO Yu-qi YANG Tian-de |
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| Abstract: | |
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| Keywords: | preconditioning mitochondrial KATP channel two-dimensional gel electrophoresis ventricular myocytes diazoxide |
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