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Protective effect and mechanism of KATP channel opener on the sinoatrial node cells of neonatal rat cultured in simulated ischemia-reperfusion*
引用本文:宋治远,仝识非,钟理.Protective effect and mechanism of KATP channel opener on the sinoatrial node cells of neonatal rat cultured in simulated ischemia-reperfusion*[J].中国人民解放军军医大学学报,2003,18(4):205-208.
作者姓名:宋治远  仝识非  钟理
作者单位:Department of Cardiology,Southwest Hospital,Third Military Medical University,Chongqing 400038,China,Department of Cardiology,Southwest Hospital,Third Military Medical University,Chongqing 400038,China,Department of Cardiology,Southwest Hospital,Third Military Medical University,Chongqing 400038,China
基金项目:Supported by National Natural Science Foundation of China (No. 30070314)
摘    要:Objective: To study the effect of KATp channel opener and its possible mechanism on the sinoatrial node cells of neonatal rats which were cultured under simulated ischemia-reperfusion. Methods: Freshly isolated sinoatrial node (SAN) cells of neonatal rats were purified and cultured for 2 d, and then they were randomly divided into the control, simulated ischemia-reperfusion group (I/R group) , group intervened with KATp channel opener pinacidil (P + I/R group), KATP Channel blocking agent 5-HD (5-HD + I/R group) , and group with the 2 agents at same time (5-HD + P + I/R group) . The survival rate of cells was measured by flow cytometry and the content of intracellular calcium in the cells of each group was detected with laser confocal microscopy. Results: ① The survival rate of SAN cells in I/R group (51. 79±6. 28)% ] was remarkably significantly lower than in control (95. 08±10. 48)% ] (P < 0.001), and very significantly lower than in P + I/R group (63. 77±5. 35) % ] (P<0.01), however, those

关 键 词:窦房结  缺血再灌注  KATP通道开放剂    新生小鼠

Protective effect and mechanism of KATP channel opener on the sinoatrial node cells of neonatal rat cultured in simulated ischemia-reperfusion
SONG Zhi-yuan TONG Shi-fei,ZHONG Li.Protective effect and mechanism of KATP channel opener on the sinoatrial node cells of neonatal rat cultured in simulated ischemia-reperfusion[J].Journal of Medical Colleges of PLA(China),2003,18(4):205-208.
Authors:SONG Zhi-yuan TONG Shi-fei  ZHONG Li
Institution:SONG Zhi-yuan TONG Shi-fei,ZHONG Li Department of Cardiology,Southwest Hospital,Third Military Medical University,Chongqing 400038,China
Abstract:
Keywords:sinoatrial node  ischemia-reperfusion  KATP channel opener  intracellular calcium
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