KGFR、P16^INK4a在新疆维吾尔族妇女宫颈上皮内瘤变及宫颈癌中的表达及意义

目的探讨角质细胞生长因子受体（keratinoeyte gowth factor reeeptor,KGFR）与P16INK4a在新疆维吾尔族妇女宫颈上皮内瘤变（cervical intraepithelial neoplasia,CIN）及宫颈鳞癌组织中的表达及意义。方法采用免疫组化链霉素抗生物素蛋白-过氧化物酶链接（SP）法检测20例慢性宫颈炎组织、70例CIN组织（CINⅠ25例、CINⅡ25例、CINⅢ20例）及40例浸润性宫颈鳞癌组织标本中KGFR、P16INK4a的表达。结果 （1）KGFR蛋白阳性表达率：浸润性宫颈鳞癌82.50%,CIN62.86%,慢性宫颈炎40.00%,差异具有统计学意义（P=0.004）;P16INK4a蛋白阳性表达率：浸润性宫颈鳞癌82.50%,CIN47.14%,慢性宫颈炎0.00%,差异具有统计学意义（P=0.000）。（2）在宫颈各组织中,KGFR蛋白与P16INK4a蛋白表达无相关性（r=0.158,P=0.073）。结论 KGFR和P16INK4a的高表达与宫颈癌的发生、发展密切相关,但是KGFR和P16INK4a无协同作用,各自以不同的方式参与了宫颈癌的发生发展。P16INK4a和KGFR检测可作为宫颈癌早期诊断和宫颈癌进展的分子指标。

Expression of KGFR and P16^INK4a protein in Xinjiang Uygur women cervical intraepithelial neoplasia and cervical cancer

Objective To investigate the expression and clinical significance of the keratinocyte growth factor receptor（KGFR/FGFR2IIIb） and P16INK4a protein in Xinjiang Uygur women cervical intraepithelial neoplasia（cervical intra-epithelial neoplasia,CIN） and cervical cancer.Methods Using immunohistochemical streptomycin avidin-peroxidase link（SP） method to detect expression of KGFR and P16INK4a protein in 20 cases of chronic cervicitis,70 cases of CIN（CIN Ⅰ 25,CIN Ⅱ 25,CIN Ⅲ 20） and 40 cases of invasive cervical squamous cell carcinoma specimens.Results（1） KGFR was followed by invasive cervical squamous cell carcinoma（82.50%）〉CIN（62.86%）〉chronic cervicitis（40.00%） and the difference is statistically significant（P=0.004）;P16INK4a protein was followed by invasive cervical squamous cell carcinoma（82.50%） 〉CIN（47.14%） 〉chronic cervicitis（0.00%） and the difference is statistically significant（P=0.000）.（2） In Various cervix uteri tissue,the expression of KGFR and P16INK4a protein was incorrelated（r=0.158,P=0.073）.Conclusion High level expressions P16INK4a and KGFR are closely related to the genesis and development of cervical cancer,but KGFR and P16INK4a have not a synergistic effect in cervical cancer,they participate in the development of cervical cancer in different ways.The detection KGFR and P16INK4a protein can be considered as a early detection index of cervical cancer and progress of cervical cancer.