亚急性氟中毒小鼠抗氧化能力及肾功能的变化

目的探讨亚急性氟中毒小鼠抗氧化能力及肾功能的变化。方法实验时间为10d,将40只昆明种小鼠随机分对照组10只和中毒组30只,雌雄各半。对照组小鼠腹腔注射生理盐水13ml/（kg.d）,中毒组小鼠腹腔注射氟化钠溶液（1.0g/L）13ml/（kg.d）。第10天剪尾取血测定血浆中谷胱甘肽过氧化物酶（GSH-Px）活性、丙二醛（MDA）含量、尿素（Urea）含量和肌酐（Cr）含量。结果中毒组与对照组相比血浆MDA含量明显升高（7.39±1.67）vs（5.89±0.81）,t=3.52,P〈0.01],GSH-Px活性和Urea含量、Cr含量三项指标比较,差异无统计学意义（426.40±50.92）vs（422.0±52.24）,（7.32±1.60）vs（6.87±2.14）]。结论本实验结果证明,亚急性氟中毒小鼠抗过氧化能力明显下降,尚未见到对肾排泄功能的严重影响。

Changes in anti-oxidation activity and renal function in mice with subacute fluorine poisoning

Objective To explore the changes of anti-oxidation activity and renal function in mice with subacute fluorine poisoning.Methods The experiment lasted for 10 days.Forty Kunming mice were randomly divided into control group（n=10）and poisoning group（n=30）.Each group included equal numbers of male and female mice.Mice in control group were intraperitoneally injected with normal saline（13 ml/kg per day）,and mice in poisoning group were intraperitoneally injected with sodium fluoride solution（1.0 g/L,13 mg/kg per day）.The tail blood of mice were collected on day 10 and the blood glutathione peroxidase activity（GSH-Px）,concentrations of malondialdehyde（MDA）,urea and creatinine（Cr） were determined.Results The MDA concentration was significantly higher in poisoning group（7.39±1.67）μmol/L]compared with that in control group（5.89±0.81）μmol/L]（P0.01）.No significant differences were observed in GSH-Px activity and concentrations of Urea and Cr between the two groups.Conclusion Subacute fluorine poisoning has negative impact on the anti-oxidation activity and no impact on renal function in mice.