中国2009年甲型H1N1流感病毒进化分析

目的研究2009年中国甲型H1N1流感病毒的进化特性和变异情况,为甲型H1N1流感的监控和防治提供科学依据。方法利用ClustaX进行序列比对,以Bioedit软件进行序列同源性分析,使用Mega 4.0软件采用邻位相连算法构建进化树,对2009年登录在Genbank数据库中的中国甲型H1N1流感病毒的HA、NA、M蛋白的氨基酸序列进行系统进化分析。结果中国2009年甲型H1N1流感病毒分离株与美国分离株A/California/04/2009（H1N1）]具有很近的亲缘关系,不同省份分离株之间的HA、NA、M蛋白相互交叉渗透,但同时各个省份间暴发的H1N1流感病毒的HA蛋白又具有一定的聚集性。香港分离株A/Hong Kong/2369/2009（H1N1）]和上海分离株A/Shanghai/LWS1/2009（H1N1）]产生了对达菲的耐药性,其他毒株仍然敏感;上海分离株A/Shanghai/LWS1/2009（H1N1）]对金刚烷胺类药物敏感,其余毒株均对其耐药。结论本研究表明,中国2009年甲型H1N1流感病毒不仅具有很强的保守性,同时又具有一定的区域特异性,毒株相对稳定,没有出现大的变异。

Phylogenetic analysis of 2009 H1N1 virus from China

Objective To analyze the characteristics of evolution and mutation of 2009 H1N1 influenza viruses from China.Methods The sequences of HA、NA、M of the viruses were download from NCBI Genbank.Multiple sequences analysis were done by the software of ClustaX,and sequence homology analysis by Bioedit,then phylogenetic trees were constructed by Mega 4.0.Results 2009 H1N1 influenza viruses from china were homologous with the strain of A/California/04/2009（H1N1） which was isolated from USA.The sequences of HA、NA、M proteins isolated from different provinces of China were reciprocal chiasmata in the phylogenetic trees,but the HA proteins were formed groups by each provinces.The phylogenetic and homology analysis suggested that the strain isolated fom Hong KongA/Hong Kong/2369/2009（H1N1）]and ShanghaiA/Shanghai/LWS1/2009（H1N1）]had devoloped a drug tolerance of Oseltamivir,and only the strain from ShanghaiA/Shanghai/LWS1/2009（H1N1）]was sensitive to adamantanamine,the others were drug resistance.Conclusion 2009 H1N1 influenza viruses from China are not only conserved but also regiospecific.The 2009 H1N1 viruses maintain stability,and no significant mutation is found.