牙周炎龈沟液中IL-6、IL-10水平变化与临床指标关系的研究

目的:探讨牙周炎病人牙周基础治疗前及治疗后6wk、8wk、10wk龈沟液(gingival crevicular fluid,GCF)中白细胞介素-6(interleukin -6,IL -6)、白细胞介素- 10(interleukin - 10,IL - 10)含量及相关临床指标的变化,为牙周炎的临床诊断和治疗提供理论依据.方法:采集40例健康人的40个健康牙位及轻度与重度牙周炎组各40个炎症牙位的GCF,采用酶联免疫吸附分析(ELISA)检测治疗前及治疗后6wk、8wk、10wkGCF中IL-6及IL - 10的含量并分别记录治疗前、后的探诊深度(probing depth,PD)、附着丧失(attachment loss,AL)、菌斑指数( plaque index,PLI)和龈沟出血指数(sulcus bleeding index,SBI),并分析其相关性.结果:(1)牙周炎组病人治疗前、后GCF中IL -6含量均显著高于健康对照组(P＜0.01),重度牙周炎组病人治疗前、后GCF中IL -6含量均高于轻度牙周炎组(P＜0.01),且牙周治疗后牙周炎组GCF中IL -6含量明显降低,并且牙周炎病人GCF 中IL -6含量与PD、AL、PLI和SBI分别呈正相关关系(P＜0.05).(2)牙周炎组病人GCF中IL - 10含量显著低于健康对照组(P＜0.01),重度牙周炎组病人GCF中IL - 10含量低于轻度牙周炎病人(P＜0.01),且牙周治疗后牙周炎组GCF中IL - 10含量明显升高,牙周炎病人GCF中IL - 10含量与PD、AL、PLI和SBI呈负相关关系(P＜0.05).(3)IL-6与IL - 10在相应时间点呈负相关关系(P＜0.05).结论:IL -6含量在牙周炎基础治疗后10wk内是逐渐降低的,而IL - 10含量在治疗后10wk内是逐渐升高的,两因子在相应的时间点呈负相关关系.

STUDY ON THE CONTENT OF IL- 6 AND IL- 10 IN GCF BEFORE AND AFTER BASIC TREATMENT OF PERIODONTITIS

Objective:To explore the changes of clinical markers and content in the Interleukin-6(IL-6),interleukin-10(IL-10)levels before and after the periodontal treatment 6,8,10wk of gingival crevicular fluid(GCF)with periodontitis.Methods:Collected gingival crevicular fluid from 40 healthy teeth with healthy people;Mild and severe periodontitis groups of 40 teeth of the GCF inflammatory.Using enzyme-linked immunosorbent assay(ELISA)to detect the IL-6 and IL-10 levelsin GCF,and record the probing depth(PD),attachment loss(CAL),plaque index(PLI)and gingival sulcus bleeding index(SBI),before and after treatment 6,8,10wk,analyzing their relevance.Results:Before and after treatment,IL-6 levels in GCF of periodontitis patients were significantly higher than healthy controls(P<0.01);IL-6 levels in GCF of severe periodontitis were higher than those with mild periodontitis group(P<0.01).After periodontal therapy,the IL-6 of periodontitis group were significantly reduced.PD,AL,PLI and SBI were positive correlation(P<0.05),while IL-10 of GCF in patients with periodontitis was significantly lower than healthy controls(P<0.01),IL-10 of GCFin patients with severe periodontitis was lower than in patients with mild periodontitis(P<0.01).After periodontal therapy,the IL-10 of periodontitis group were significantly increased.PD,AL,PLI and SBI was negatively correlated(P<0.05),IL-6 and IL-10 are in the corresponding negative correlation between time points(P<0.05).Conclusion: IL-6 levels after treatment is a gradual decrease in 10wk,while the IL-10 levels is gradually increased in 10wk,in the corresponding time point the two factors IL-6 and IL-10 are negative correlation.