| 抗癌生物活性肽对实验性胃癌PCNA、caspase-8基因表达的影响 |
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| 引用本文： | 王玉芳,闫美荣,贾淑芹,刘桂荣,苏秀兰.抗癌生物活性肽对实验性胃癌PCNA、caspase-8基因表达的影响[J].内蒙古医学院学报,2009,31(1). |
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| 作者姓名： | 王玉芳 闫美荣 贾淑芹 刘桂荣 苏秀兰 |
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| 作者单位： | 1. 内蒙古医学院附属医院,病理科,内蒙古,呼和浩特,010050
2. 内蒙古医学院,临床医学研究中心 |
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| 摘 要： | 目的:探讨抗癌生物活性肽作用于荷胃癌裸鼠后,对其增殖及凋亡相关基因表达变化的影响.方法:将裸鼠接种胃癌MGC-803细胞后使之致瘤.实验随机分为3组,对照组11只,脾肽实验组12只,肝肽实验组11只;分别尾静脉注射生理盐水0.4mL、脾肽0.4mL(0.15mg/mL)、肝肽0.4mL(0.05mg/mL).连续处理35d,停药4d后处死裸鼠,取肿瘤,以4%多聚甲醛固定,常规石蜡包埋,4μm切片.应用免疫组化SABC法检测PCNA、caspase-8蛋白在实验组与对照组中的表达情况.结果:PCNA蛋白在对照组(NS组)肿瘤中阳性表达较强,在P组、G组裸鼠肿瘤中PCNA蛋白的表达均比对照组的表达弱,且有显著性差异(P<0.01);caspas-8蛋白在对照组(NS组)肿瘤中阳性表达较弱,P、G组裸鼠肿瘤中caspas-8蛋白的表达均比对照组表达强,且有显著性差异(P<0.01).结论:抗癌生物活性肽能降低胃癌移植瘤细胞的增殖活性,并能促进其凋亡.
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| 关 键 词： | 胃癌 裸鼠 增殖 凋亡 抗癌生物活性肽 |
INFLUENCE OF THE PCNA AND CASPASE-8 EXPRESSION OF ANTI-CANCER BIOACTIVE PEPTIDE ON EXPERIMENTAL GASTRIC CANCER |
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| WANG Yu-fang,YAN Mei-rong,JIA Shu-qin,et al..INFLUENCE OF THE PCNA AND CASPASE-8 EXPRESSION OF ANTI-CANCER BIOACTIVE PEPTIDE ON EXPERIMENTAL GASTRIC CANCER[J].Acta Academiae Medicinae Neimongol,2009,31(1). |
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| Authors: | WANG Yu-fang YAN Mei-rong JIA Shu-qin |
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| Institution: | WANG Yu-fang,YAN Mei-rong,JIA Shu-qin,et al.(Department of Pathology,Affiliated Hospital,Inner Mongolia Medical College,Hohhot 010050 China) |
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| Abstract: | Objective:To investigate the expression changes of proliferating and apoptotic related genes of anti-cancer bioactive peptide treated tumor-loaded nude mice.Methods:Gastric cancer cell line MGC-803 was inoculated to nude mice to make them tumor-loaded.Nude mice were divided into 3 groups randomly:11 as control group,12 as spleen peptide group and 11 as liver peptide group.3 groups of nude mice were administered with 0.4mL normal saline,0.4mL spleen peptide(0.15mg/mL)and 0.4mL liver peptide(0.05mg/mL)continu... |
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| Keywords: | gastric cancer nude mice proliferation apoptosis anti cancer bioactive peptide |
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