骨松灵合剂对去卵巢大鼠骨组织雌激素受体表达的影响

目的探讨骨松灵合剂对去卵巢大鼠骨组织雌激素受体（ER）表达的影响。方法本实验采用免疫组织化学方法检测雌性大鼠去卵巢后骨组织中ER的变化，以及骨松灵合剂、雌激素治疗后对其产生的影响。结果椎体松质骨中ERα、ERB主要分布于骨小梁周围的成骨细胞、破骨细胞及骨髓基质细胞中，阳性颗粒为棕黄色，胞浆胞核均有表达，但胞核着色较深，与假手术组（sham组）（ERα：140．6±5．7；ERB：118．6±6．5）比较，去卵巢对照组（OVX组）（ERα：119．2±7．1；ERβ：80．8±3．7）大鼠骨小梁周围及骨髓中阳性细胞灰度明显降低（P〈0．01），而中药组（ERα：137．5±4．9；ERβ：113．8±7．4）、E2组（ERα：143．8±6．2；ERβ：116．6±4．8）阳性细胞灰度与OVX组相比显著增加（P〈0．01），中药组与E2组相比较差异无明显统计学意义（P〉0．05）。结论骨松灵合剂可以上调大鼠去卵巢后骨组织中ERα、ERβ表达，促进骨形成，可能是治疗骨质疏松症的重要机制。

Influence of gusongling mixture on expression of estrogen receptor in bone tissue of ovariectomy rats

Objective To explore the influence of Gusongling mixture on the expression of estrogen receptor in bone tissue of ovariectomy rats. Methods This experiment adopted Immunohistochemistry approaches to examine the variety of ER in the bone tissue, and the influence of Gusongling mixture or estrogen on it after the treatment. Results ERα ,ERβ of centrum spongy bone were distributed in surroundings of bone trabecula, osteoblasts,osteoclasts and marrow matrix. ,positive granules were buffy, they are in both cytoplasma and mesoplast ,but granules in mesoplast dyed deep, spa of positive cell of surroundings of bone trabecula and marrow reduced significantly in OVX group compared with SHAM group（ P〈0.01 ）. But spa of positive cell increased obviously in the traditional Chinese medicine group and E2 group compared with OVX group（P〈0.01）. The traditional Chinese medicine group and E2 group compared with no significant statistical difference（P〉0.05 ）. Conclusion ERα ,ERβ took into action together in metabolize modulation of bone. Gusongling mixture can reverse the expression of ERα, ERβin bone tissue of ovariectomy famale rats, and promoted the bone formation,thus attained to therapy function.