糖基化终末产物在糖尿病大鼠骨质疏松中的作用及阿司匹林干预性观察

目的观察晚期糖基化终末产物（advanced glycation end-products,AGEs）与糖尿病骨质疏松大鼠骨密度（BMD）、骨生物力学指标的相关性,及阿司匹林（Aspirin ASP）干预作用。方法12周龄雄性SD大鼠36只,随机抽取8只作为对照组N,余28只作糖尿病建模,成模大鼠随机分为糖尿病无干预组（DM n=14）、阿司匹林干预组（ASP n=14）。建模成功后喂养16周处死所有大鼠,取左股骨检测BMD,L3椎体行生物力学试验,右胫骨测AGEs含量。结果DM组骨组织AGEs与N组比较显著增高（P〈0.01）,也明显高于ASP组（P〈0.01）;DM组大鼠AGEs与BMD、骨生物力学指标呈显著性负相关（P〈0.01或P〈0.05）;DM组BMD,骨生物力学指标均降低N组及ASP组,差异具有显著性（P〈0.01或P〈0.05）。结论提示AGEs参与了糖尿病大鼠骨质疏松的发生发展,ASP对糖尿病骨质疏松具有一定的干预作用。

The study on ASP's intervention to BMD and bone biomechanics of AGEs-related osteoporosis in diabetic rats

Objective To investigate the role of AGEs in the pathogenesis of diabetic osteoporosis and the effect of ASP＇s intervention combineing with the changes of bone mineral density,bio-mechanical indicators and markers of bone metabolism.Methods Thirty-six healthy male SD rats with twelve-weeks-old were randomly divided into the control（n=8） and the STZ-induced diabetic groups（n=28）.After the success of induction.The rats was randomly divided into intervention group（DM n=14）,Aspirin intervention group（ASA n=14）.All rats were sacrificed sixteen weeks after setting up model successfully.Measured the BMD of left femur,and AGEs content of the right tibia.The L3 vertebral body for biomechanical testing.Results There was a significantly negatively correlation between AGEs from BMD and Bone biomechanics.Compared with the control group N and ASP,the changes of Bone biomechanical and BMD were significant（P 0.01 or P 0.05）.Conclusion AGEs involved in the occurrence and development of osteoporosis in diabetic rats.Aspirin has some intervention effect on the bone metabolism disorder of Diabetic rats.