苯那普利和缬沙坦联合应用对肾病大鼠肾脏Ⅳ型胶原和纤维连接蛋白的影响

目的探讨苯那普利和缬沙坦联合应用对肾病大鼠细胞外基质Ⅳ型胶原和纤维连接蛋白的影响。方法55只雄性SD大鼠,分为对照组、模型组、苯那普利组、缬沙坦组、联合组。后4组在单侧肾切除1周后给予阿霉素5 mg.kg-1尾静脉注射。苯那普利组给予苯那普利6 mg.kg-1.d-1灌胃,缬沙坦组给予缬沙坦20 mg.kg-1.d-1灌胃,联合组给予苯那普利3 mg.kg-1.d-1联合缬沙坦10mg.kg-1.d-1灌胃。12周后用免疫组化检测肾脏Ⅳ型胶原和纤维连接蛋白的表达。结果肾脏Ⅳ型胶原的表达在苯那普利组、缬沙坦组、联合组均较模型组明显减少(P均<0.01),而联合组又低于苯那普利组和缬沙坦组(P均<0.05)。纤维连接蛋白的表达在苯那普利组、缬沙坦组、联合组均较模型组明显减少(P均<0.01),联合组与苯那普利组和缬沙坦组无显著性差异(P>0.05)。结论苯那普利和缬沙坦联合治疗能进一步减少肾脏Ⅳ型胶原的表达,延缓肾小球硬化,联合治疗的疗效优于单独应用。

Effects of the Combination Treatment with Benazepril and Valsartan on Collagen Ⅳ and Fibronectin in the Kidney of Nephritic Rats

Objective To explore the effects of the combination treatment with benazepril and valsartan on collagen Ⅳand fibronectin(FN)in the kidney of nephritic rats.Methods Fifty-five male Sprague-Dawley rats were divided into control group,model group,benazepril treated group,valsartan treated group and co-treated group.The rats from later four groups were cut off one side kidney and injected with adriamycin(5 mg·kg~(-1)) through tail vein one week later.The rats from later three treated groups were respectively gastric-perfused with benazepril(6mg·kg~(-1)),valsantan(20 mg·kg~(-1)),both benazepril(3 mg·kg~(-1)) and valsantan(10 mg·kg~(-1)) every day for 12 weeks.Immunhistochemistry was utilized to detect the expression of collagen Ⅳand FN in the kidney.Results The expression of collagen Ⅳ in benazepril treated group,valsartan treated group and co-treated group was significantly decreased(P<0.01,respectively), while that in co-treated group was lower than that in benazepril and valsartan group(P<0.05,respectively).The expression of FN in benazepril treated group and valsartan treated group and co-treated group was significantly reduced,but there was no significantly difference among co-treated group and benazepril and valsartan group.Conclusion The combination of the treatment with benazepril and valsartan can more reduce the expression of collagen Ⅳ in kidney and postpone development of glonerulosclerosis.The effect of combination treatment with benazepril and valsartan is superior to monotherapy.