高效液相色谱法测定不同产地白芷中欧前胡素和异欧前胡素含量 |
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引用本文: | 张慧,海广范,尹志奎,聂红.高效液相色谱法测定不同产地白芷中欧前胡素和异欧前胡素含量[J].新乡医学院学报,2009,26(2). |
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作者姓名: | 张慧 海广范 尹志奎 聂红 |
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作者单位: | 1. 新乡医学院药学院药理学教研室,河南,新乡,453003 2. 暨南大学药学院中药药理学教研室,广东,广州,510632 |
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基金项目: | 新乡医学院高学历人才资助项目 |
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摘 要: | 目的采用高效液相色谱法(HPLC)同时测定不同产地白芷中欧前胡素和异欧前胡素的含量,为更好的评价不同产地白芷的质量提供依据。方法色谱柱:L ichrospher-C18(250×4.6 mm,5μm),柱温:30℃,流动相:甲醇-水,梯度洗脱,流速为1.0 mL.m in-1。结果欧前胡素、异欧前胡素进样量分别在0.54~5.40μg、0.244~2.44μg范围内,呈现良好的线性关系。欧前胡素平均回收率99.020%,相对标准偏差(RSD)3.318%;异欧前胡素平均回收率97.528%,RSD 2.904%。欧前胡素平均含量为1.465 mg.g-1;异欧前胡素平均含量为0.575 mg.g-1。结论该测定方法简单,快速,重现性好,可作为该药材质量的控制方法。
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关 键 词: | 白芷 高效液相色谱法 欧前胡素 异欧前胡素 |
Determination of contents of imperatorin and isoimperatorin in Radix Angelicae Dahuricae from different areas by HPLC |
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Abstract: | Objective To develop a method for the determination of imperatorin and isoimperatorin in Radix Angelicae Dahuricae.Methods A HPLC method was established.Lichrospher-C18 column(250 mm×4.6 mm,5 μm)was used,with mixture of methanol and water in a gradient mode.The flow rate was 1.0 mL·min-1.Results The calibration of imperatorin was linear in range of 0.54~5.40 μg.The average recovery was 99.020%,RSD 3.318%.The calibration of isoimperatorin was linear in range of 0.244~2.44 μg.The average recovery was 97.528%,RSD 2.904%.The average contents of imperatorin and isoimperatorin were 1.465 mg·g-1 and 0.575 mg·g-1 respectively.Conclusion The method is appropriate for the detemination of Radix Angelicae Dahuricae. |
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Keywords: | Radix Angelicae Dahuricae high performance liquid chromatography imperatorin isoimperatorin |
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