苯酚-硫酸法测定河南丝瓜络多糖的含量

目的测定河南产丝瓜络多糖的含量,探讨其药用价值和开发前景。方法采用苯酚-硫酸法,以葡萄糖(105℃干燥至恒重)为对照品测定河南丝瓜络中多糖的含量。结果在6~91μg.mL-1的范围内,样品的浓度与吸光度有良好的线性关系,线性回归方程A=8.995 8 c-0.007 8(r=0.999 7),加标回收率101.8%(n=5,相对标准偏差=1.59%),河南丝瓜络多糖的平均含量为7.66%。结论河南产丝瓜络的多糖含量高于广东、山西和浙江等产地,具有较高的药用价值和开发前景。

Determination of the content of polysaccharide in Retinervus luffac fructus produced in Henan province with phenol-sulfuric acid

Objective To determine the content of polysaccharide in retinervus luffac fructus produced in Herren province and to explore the medical value and developing perspective. Methods The content of polysaccharide was determined with the method of phenol-sulfuric acid, and the glucose（ drying at 105 ℃ to keep constant weight） was used as reference substance. Restilts The sample concentration was good linear relationship with absorbance within the range of 6 - 91 μg·mL^-1, the equation of linear regression is A = 8. 995 8c-0.007 8 （ r = 0.999 7 ） , the average recovery was 101.8 % （ n = 5, RSD = 1.59 % ） and the average concentration of polysaccharide was 7.66%. Conclusion The content of polysaccharide in Retinervus luffac fruetus produced in Henan is higher than in Guangdong, Shanxi, Zhejiang and other provinces, and it has higher medical value and development foreground.