递增浓度肿瘤坏死因子攻击下肺泡上皮细胞分泌IL-8水平变化

目的观察不同浓度肿瘤坏死因子攻击下肺泡上皮细胞(A549细胞)存活率和分泌IL-8水平变化。方法A549细胞分为对照组和实验组,分别采用不同浓度TNF-α攻击,培养24h后测定各组细胞存活率、上清IL-8水平。结果不同浓度TNF-α攻击均能明显抑制A549细胞生长,其中5000u·ml-1浓度组细胞存活率降为(90.1±1.1)%。TNF-α攻击浓度由100u·ml-1升至1000u·ml-1时,A549细胞分泌IL-8量由9.1±3.5pg·ml-1升至203.7±12.4pg·ml-1,TNF-α攻击浓度超过5000u·ml-1后IL-8分泌量开始下降。结论以100u·ml-1~1000u·ml-1之间浓度的TNF-α进行攻击,A549细胞IL-8分泌量与TNF-α浓度存在剂量-效应关系,反映了急性肺损伤/急性呼吸窘迫综合征状态下肺泡上皮细胞分泌炎性介质情况。

Release of interleukin-8 from alveolar epithelial cells induced by different concentration tumor necrosis factor-alpha in vitro

Objective To investigate the effect of tumor necrosis factor-alpha on the secretion of interleukin-8(IL-8) from alveolar epithelial cells(A549) in vitro.Methods A549 cells were divided into five groups: one control group and four TNF-α groups.After 24 hours' culture,the livability of A549 cells and the secretion of IL-8 were measured by ELISA.Results The livability of TNF-α groups were lower than that of the control group (5000 u·ml~ -1 -TNF-α group) (90.1±1.1)% vs control group (97.9±0.9)%.TNF-α induced a rapidly growing of interleukin-8 in the TNF-α group ( 1000u·ml~ -1 -TNF-α group 203.7±12.4 pg·ml~ -1 vs control group 9.1±3.5 pg·ml~ -1 ). Conclusion When the concentration of TNF-α was 100 u·ml~ -1 ～1000 u·ml~ -1 ,there was a dose-dependent fashion between the secretion IL-8 and the concentration of TNF-α,which reflects the state of alveolar type II epithelial cell in acute luing injury/acute respiratory disease syndrome.