首页 | 本学科首页   官方微博 | 高级检索  
检索        

人脐带间充质干细胞裂解液在体外对食管癌EC9706细胞增殖与迁移的抑制作用
引用本文:刘富磊,齐 博,赵宝生,姚文建,李汉臣,白 玉,钟根深.人脐带间充质干细胞裂解液在体外对食管癌EC9706细胞增殖与迁移的抑制作用[J].新乡医学院学报,2014(4):248-252.
作者姓名:刘富磊  齐 博  赵宝生  姚文建  李汉臣  白 玉  钟根深
作者单位:[1]新乡医学院,河南新乡453003 [2]新乡医学院第一附属医院胸外科,河南卫辉453100 [3]新乡医学院病理学教研室,河南新乡453003 [4]河南省神经病学研究所,河南卫辉453100
基金项目:河南省科技厅资助项目(编号:112300410163)
摘    要:目的探讨人脐带间充质干细胞(hUCMSCs)裂解液在体外对食管癌EC9706细胞增殖、迁移的抑制作用。方法用组织块培养法体外培养hUCMSCs,流式细胞仪检测细胞表型;收集hUCMSCs,并用反复冻融法制备hUCMSCs裂解液;不同浓度hUCMSCs裂解液作用于食管癌EC9706细胞一定时间,采用四甲基偶氮唑盐(MTT)法检测细胞增殖状况,倒置显微镜观察细胞形态。通过Tranwell迁移实验观察hUCMSCs裂解液对食管癌EC9706细胞迁移能力的影响。结果人脐带组织块培养712 d后可见贴壁的成纤维样细胞,流式细胞仪分析显示第5代细胞均表达CD29、CD90和CD166,不表达CD34、CD45和人类白细胞抗原-DR。MTT法显示加入hUCMSCs数等于或大于食管癌细胞数的裂解液时,与对照组比较明显抑制食管癌细胞的生长(P<0.05),且实验组各组抑制食管癌细胞增殖能力差异无统计学意义(P>0.05)。Tranwell迁移实验显示,实验组EC9706细胞的迁移较对照组明显受到抑制(P<0.05)。结论 hUCMSCs裂解液对食管癌细胞EC9706的增殖和迁移均有抑制作用。

关 键 词:人脐带间充质干细胞  食管癌  增殖  迁移

Suppressive effect of human umbilical cord mesenchymal stem cells lysate on proliferation and migration of esophageal cancer EC9706 cells in vitro
LIU Fu-lei,QI Bo,ZHAO Bao-sheng,YAO Wen-jian,LI Han-chen,BAI Yu,ZHONG Gen-shen.Suppressive effect of human umbilical cord mesenchymal stem cells lysate on proliferation and migration of esophageal cancer EC9706 cells in vitro[J].Journal of Xinxiang Medical College,2014(4):248-252.
Authors:LIU Fu-lei  QI Bo  ZHAO Bao-sheng  YAO Wen-jian  LI Han-chen  BAI Yu  ZHONG Gen-shen
Institution:1. Xinxiang Medical University ,Xinxiang 453003, Henan Province, China;2. Department of Thoracic Surgery, the First Affilia- ted Hospital of Xinxiang Medical University, Weihui 453100, Henan Province, China ; 3. Department of Pathology Division, X inx- iang Medical University ,Xinxiang 453003, Henan Province, China;4. Neurology Institute of Henan Province, Weihui 453100, Henan Province, China)
Abstract:Objective To investigate the suppressive effect of human umbilical cord mesenchymal stem cells( hUCMSCs) lysate on proliferation and migration of esophageal cancer EC9706 cells in vitro. Methods hUCMSCs was cultured by using tissue culture in vitro and cell phenotype analysis was performed by using flow cytometry. hUCMSCs were collected and hUCMSCs lysate was prepared through repeated freezing and thawing. Different concentration of lysate were added to esophageal cancer EC9706 cells culturing for a period of time,cell proliferation was evaluated with 3-( 4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide( MTT) assay and cell morphology was observed by using inverted microscope. Tranwell experiment was exerted to observe the effect of hUCMSCs lysate on the migration of EC9706 cell. Results When hUCMSCs was cultured for 7- 12 days,adherent and fibroblast-like cells were visible. Flow cytometry analysis revealed that CD29,CD90 and CD166 were highly expressed on the plasma membrane of the 5thgeneration cells,but negative for CD34,CD45 and human leucocyte antigen-DR. MTT assay showed that lysate obviously inhibited the growth of esophageal cancer cells compared with the control group when the adding number of hUCMSCs was equal to or greater than the number of esophageal cancer cells( P〈0. 05),and the inhibition of cell proliferation of each experimental group in esophageal cancer had no significant difference( P〈0. 05). Tranwell experiment showed that the experimental group EC9706 cell migration was significantly inhibited compared with the control group( P〈0. 05). Conclusion hUCMSCs lysate has an inhibition effect on both proliferation and migration of esophageal cancer EC9706 cells in vitro.
Keywords:human umbilical cord mesenchymal stem cells  esophageal cancer  proliferation  migration
本文献已被 CNKI 维普 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号